Combined Transcriptomic and Metabolomic Analysis Reveals Insights into Resistance of Arabidopsis Mutant Against the Phytopathogenic Fungus

Overview

Journal Plants (Basel)

Date 2022 Dec 23

PMID 36559570

Authors

Eleni Kalogeropoulou

Konstantinos A Aliferis

Sotirios E Tjamos

Irene Vloutoglou

Epaminondas J Paplomatas

Affiliations

Soon will be listed here.

Abstract

The wilt-inducing strains of are responsible for severe damage to many economically important plant species. The most cost-effective and environmentally safe method for the management of Fusarium wilt is the use of resistant cultivars when they are available. In the present study, the Arabidopsis genotype with disruptions in the () gene, which encodes the major hydrolytic enzyme that degrades starch to maltose, had significantly lower susceptibility to f. sp. () compared to wild-type (wt) plants. It showed the lowest disease severity and contained reduced quantities of fungal DNA in the plant vascular tissues when analyzed with real-time PCR. Through metabolomic analysis using gas chromatography (GC)-mass spectrometry (MS) and gene-expression analysis by reverse-transcription quantitative PCR (RT-qPCR), we observed that defense responses of Arabidopsis mutants are associated with starch-degradation enzymes, the corresponding modification of the carbohydrate balance, and alterations in sugar (glucose, sucrose, trehalose, and myo-inositol) and auxin metabolism.

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