Growth of a Rat Neuroblastoma Cell Line in Serum-free Supplemented Medium

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1979 Jan 1

PMID 284369

Citations 514

Authors

J E Bottenstein

G H Sato

Affiliations

Soon will be listed here.

Abstract

The rat neuroblastoma B104 cell line, which originated in the central nervous system, was able to proliferate in the absence of serum in synthetic medium supplemented with insulin, transferrin, progesterone, selenium, and putrescine. When added individually, each supplement had little or no effect; however, in combination there was a marked synergistic effect on cell number. The cells attained the same saturation density in this medium as in medium with 10% fetal calf serum added. More extensive process formation was observed in the supplemented medium, and other differentiated properties were retained as well.

Citing Articles

Neurospheres from Ovarian Cortical Cells in Mammals: Generation In Vitro and Neurosphere Assay.

Gonzalez-Gil A, Picazo R Methods Mol Biol. 2025; 2899:67-87.

PMID: 40067618 DOI: 10.1007/978-1-0716-4386-0_6.

Defined media reveals the essential role of lipid scavenging to support cancer cell proliferation.

Newsom O, Sullivan L bioRxiv. 2025; .

PMID: 40027810 PMC: 11870423. DOI: 10.1101/2025.02.12.637975.

Proof-of-Concept for Long-Term Human Endometrial Epithelial Organoids in Modeling Menstrual Cycle Responses.

Jiang Y, Palomares A, Munoz P, Nalvarte I, Acharya G, Inzunza J Cells. 2024; 13(21.

PMID: 39513919 PMC: 11545391. DOI: 10.3390/cells13211811.

Modeling Epithelial Homeostasis and Perturbation in Three-Dimensional Human Esophageal Organoids.

Shimonosono M, Morimoto M, Hirose W, Tomita Y, Matsuura N, Flashner S Biomolecules. 2024; 14(9).

PMID: 39334892 PMC: 11430971. DOI: 10.3390/biom14091126.

The WMI Rat of Premature Cognitive Aging Presents Intrinsic Vulnerability to Oxidative Stress in Primary Neurons and Astrocytes Compared to Its Nearly Isogenic WLI Control.

Ferreira A, Harter A, Afreen S, Kanai K, Batori S, Redei E Int J Mol Sci. 2024; 25(3).

PMID: 38338968 PMC: 10855588. DOI: 10.3390/ijms25031692.

References

Evans V, BRYANT J, FIORAMONTI M, McQUILKIN W, SANFORD K, Earle W . Studies of nutrient media for tissue cells in vitro. I. A protein-free chemically defined medium for cultivation of strain L cells. Cancer Res. 1956; 16(1):77-86. View

HERBST E, Snell E . Putrescine and related compounds as growth factors for Hemophilus parainfluenzae 7991. J Biol Chem. 1949; 181(1):47-54. View

Evans V, BRYANT J, Kerr H, SCHILLING E . CHEMICALLY DEFINED MEDIA FOR CULTIVATION OF LONG-TERM CELL STRAINS FROM FOUR MAMMALIAN SPECIES. Exp Cell Res. 1964; 36:439-74. DOI: 10.1016/0014-4827(64)90302-7. View

Havrankova J, Roth J, Brownstein M . Insulin receptors are widely distributed in the central nervous system of the rat. Nature. 1978; 272(5656):827-9. DOI: 10.1038/272827a0. View

Rudland P, Durbin H, Clingan D, de Asua L . Iron salts and transferrin are specifically required for cell division of cultured 3T6 cells. Biochem Biophys Res Commun. 1977; 75(3):556-62. DOI: 10.1016/0006-291x(77)91508-x. View

Bottenstein J, Hayashi I, Hutchings S, Masui H, Mather J, McClure D . The growth of cells in serum-free hormone-supplemented media. Methods Enzymol. 1979; 58:94-109. DOI: 10.1016/s0076-6879(79)58127-0. View

Rizzino A, Sato G . Growth of embryonal carcinoma cells in serum-free medium. Proc Natl Acad Sci U S A. 1978; 75(4):1844-8. PMC: 392437. DOI: 10.1073/pnas.75.4.1844. View

Czech M . Molecular basis of insulin action. Annu Rev Biochem. 1977; 46:359-84. DOI: 10.1146/annurev.bi.46.070177.002043. View

POHJANPELTO P . Putrescine transport is greatly increased in human fibroblasts initiated to proliferate. J Cell Biol. 1976; 68(3):512-20. PMC: 2109648. DOI: 10.1083/jcb.68.3.512. View

10.

Hutchings S, Sato G . Growth and maintenance of HeLa cells in serum-free medium supplemented with hormones. Proc Natl Acad Sci U S A. 1978; 75(2):901-4. PMC: 411365. DOI: 10.1073/pnas.75.2.901. View

11.

Guilbert L, Iscove N . Partial replacement of serum by selenite, transferrin, albumin and lecithin in haemopoietic cell cultures. Nature. 1976; 263(5578):594-5. DOI: 10.1038/263594a0. View

12.

Phillips M, COXON R . Effect of insulin and phenobarbital on uptake of 2-deoxyglucose by brain slices and hemidiaphragms. J Neurochem. 1976; 27(2):643-5. DOI: 10.1111/j.1471-4159.1976.tb12299.x. View

13.

Takaoka T, Katsuta H . Long-term cultivation of mammalian cell strains in protein- and lipid-free chemically defined synthetic media. Exp Cell Res. 1971; 67(2):295-304. DOI: 10.1016/0014-4827(71)90412-5. View

14.

JIMENEZ DE ASUA L, Surian E, Flawia M, Torres H . Effect of insulin on the growth pattern and adenylate cyclase activity of BHK fibroblasts. Proc Natl Acad Sci U S A. 1973; 70(5):1388-92. PMC: 433503. DOI: 10.1073/pnas.70.5.1388. View

15.

POHJANPELTO P, Raina A . Identification of a growth factor produced by human fibroblasts in vitro as putrescine. Nat New Biol. 1972; 235(60):247-9. DOI: 10.1038/newbio235247a0. View

16.

Schubert D, Heinemann S, Carlisle W, Tarikas H, Kimes B, Patrick J . Clonal cell lines from the rat central nervous system. Nature. 1974; 249(454):224-7. DOI: 10.1038/249224a0. View

17.

Lajtha A, Sershen H . Substrate specificity of uptake of diamines in mouse brain slices. Arch Biochem Biophys. 1974; 165(2):539-47. DOI: 10.1016/0003-9861(74)90280-x. View

18.

Temin H . Studies on carcinogenesis by avian sarcoma viruses. VI. Differential multiplication of uninfected and of converted cells in response to insulin. J Cell Physiol. 1967; 69(3):377-84. DOI: 10.1002/jcp.1040690314. View

19.

STADTMAN T . Selenium biochemistry. Science. 1974; 183(4128):915-22. DOI: 10.1126/science.183.4128.915. View

20.

Schubert D, Carlisle W, Look C . Putative neurotransmitters in clonal cell lines. Nature. 1975; 254(5498):341-3. DOI: 10.1038/254341a0. View