Proton/electron Stoichiometry of Mitochondrial Complex I Estimated from the Equilibrium Thermodynamic Force Ratio

Overview

Journal Biochem J

Specialty Biochemistry

Date 1988 Jun 1

PMID 2843170

Citations 28

Authors

G C Brown

M D Brand

Affiliations

Soon will be listed here.

Abstract

The thermodynamic forces on electrons (delta Eh) and protons (delta p) across mitochondrial complexes I, III and IV were measured in isolated mitochondria respiring on succinate. The force ratio (delta Eh/delta p) across complex I close to equilibrium was found to be about 2. The equilibrium force ratio across complex I was measured during sulphite oxidation and was again close to 2. These results indicate that the proton/electron stoichiometry of complex I is 2, in conditions of high protonmotive force.

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