Semi-automatic Synthesis and Biodistribution of N-(2-F-fluoropropionyl)-bis(zinc (II)-dipicolylamine) (F-FP-DPAZn2) for AD Model Imaging

Overview

Journal BMC Med Imaging

Publisher Biomed Central

Specialty Radiology

Date 2017 Apr 23

PMID 28431519

Authors

Fuhua Wen

Dahong Nie

Kongzhen Hu

Ganghua Tang

Shaobo Yao

Caihua Tang

Affiliations

Soon will be listed here.

Abstract

Background: Phosphatidylserine (PS)-targeting positron emission tomography (PET) imaging with labeled small-molecule tracer is a crucial non-invasive molecule imaging method of apoptosis. In this study, semi-automatic radiosynthesis and biodistribution of N-(2-F-fluoropropionyl)-bis(zinc(II)-dipicolylamine) (F-FP-DPAZn2), as a potential small-molecule tracer for PET imaging of cell death in Alzheimer's disease (AD) model, were performed.

Methods: F-FP-DPAZn2 was synthesized on the modified PET-MF-2V-IT-I synthesizer. Biodistribution was determined in normal mice and PET images of AD model were obtained on a micro PET-CT scanner.

Results: With the modified synthesizer, the total decay-corrected radiochemical yield of F-FP-DPAZn2 was 35 ± 6% (n = 5) from F within 105 ± 10 min. Biodistribution results showed that kidney has the highest uptake of F-FP-DPAZn2. The uptake of radioactivity in brain kept at a relatively low level during the whole observed time. In vivo F-FP-DPAZn2 PET images demonstrated more accumulation of radioactivity in the brain of AD model mice than that in the brain of normal mice.

Conclusions: The semi-automatic synthetic method provides a slightly higher radiochemical yield and shorter whole synthesis time of F-FP-DPAZn2 than the manual operation method. This improved method can give enough radioactivity and high radiochemical purity of F-FP-DPAZn2 for in vivo PET imaging. The results show that F-FP-DPAZn2 seems to be a potential cell death tracer for AD imaging.

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