Optical Imaging of Bacterial Infection in Living Mice Using a Fluorescent Near-infrared Molecular Probe

Overview

Journal J Am Chem Soc

Publisher American Chemical Society

Specialty Chemistry

Date 2006 Dec 21

PMID 17177377

Citations 70

Authors

W Matthew Leevy

Seth T Gammon

Hua Jiang

James R Johnson

Dustin J Maxwell

Erin N Jackson

Manuel Marquez

David Piwnica-Worms

Bradley D Smith

Affiliations

Soon will be listed here.

Abstract

An optical imaging probe was synthesized by attaching a near-infrared carbocyanine fluorophore to an affinity group containing two zinc(II) dipicolylamine (Zn-DPA) units. The probe has a strong and selective affinity for the surfaces of bacteria, and it was used to image infections of Gram-positive S. aureus and Gram-negative E. coli bacteria in living nude mice. After intravenous injection, the probe selectively accumulates at the sites of localized bacterial infections in the thigh muscles of the mice.

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