High-Throughput and Cost-Effective Characterization of Induced Pluripotent Stem Cells

Overview

Journal Stem Cell Reports

Publisher Cell Press

Specialty Cell Biology

Date 2017 Apr 16

PMID 28410643

Citations 30

Authors

Matteo DAntonio

Grace Woodruff

Jason L Nathanson

Agnieszka DAntonio-Chronowska

Angelo Arias

Hiroko Matsui

Roy Williams

Cheryl Herrera

Sol M Reyna

Gene W Yeo

Lawrence S B Goldstein

Athanasia D Panopoulos

Kelly A Frazer

Affiliations

Soon will be listed here.

Abstract

Reprogramming somatic cells to induced pluripotent stem cells (iPSCs) offers the possibility of studying the molecular mechanisms underlying human diseases in cell types difficult to extract from living patients, such as neurons and cardiomyocytes. To date, studies have been published that use small panels of iPSC-derived cell lines to study monogenic diseases. However, to study complex diseases, where the genetic variation underlying the disorder is unknown, a sizable number of patient-specific iPSC lines and controls need to be generated. Currently the methods for deriving and characterizing iPSCs are time consuming, expensive, and, in some cases, descriptive but not quantitative. Here we set out to develop a set of simple methods that reduce cost and increase throughput in the characterization of iPSC lines. Specifically, we outline methods for high-throughput quantification of surface markers, gene expression analysis of in vitro differentiation potential, and evaluation of karyotype with markedly reduced cost.

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