Electroformation of Giant Unilamellar Vesicles on Stainless Steel Electrodes

Overview

Journal ACS Omega

Publisher American Chemical Society

Specialty Chemistry

Date 2017 Apr 11

PMID 28393132

Citations 8

Authors

Valerio Pereno

Dario Carugo

Luca Bau

Erdinc Sezgin

Jorge Bernardino de la Serna

Christian Eggeling

Eleanor Stride

Affiliations

Soon will be listed here.

Abstract

Giant unilamellar vesicles (GUVs) are well-established model systems for studying membrane structure and dynamics. Electroformation, also referred to as electroswelling, is one of the most prevalent methods for producing GUVs, as it enables modulation of the lipid hydration process to form relatively monodisperse, defect-free vesicles. Currently, however, it is expensive and time-consuming compared with other methods. In this study, we demonstrate that 1,2-dioleoyl--glycero-3-phosphatidylcholine GUVs can be prepared readily at a fraction of the cost on stainless steel electrodes, such as commercially available syringe needles, without any evidence of lipid oxidation or hydrolysis.

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