Nanoindentation Analysis of the Micromechanical Anisotropy in Mouse Cortical Bone

Overview

Journal R Soc Open Sci

Specialty Science

Date 2017 Apr 8

PMID 28386450

Citations 12

Authors

Michele Casanova

Anna Balmelli

Davide Carnelli

Diana Courty

Philipp Schneider

Ralph Muller

Affiliations

Soon will be listed here.

Abstract

Studies investigating micromechanical properties in mouse cortical bone often solely focus on the mechanical behaviour along the long axis of the bone. Therefore, data on the anisotropy of mouse cortical bone is scarce. The aim of this study is the first-time evaluation of the anisotropy ratio between the longitudinal and transverse directions of reduced modulus and hardness in mouse femurs by using the nanoindentation technique. For this purpose, nine 22-week-old mice (C57BL/6) were sacrificed and all femurs extracted. A total of 648 indentations were performed with a Berkovich tip in the proximal (P), central (C) and distal (D) regions of the femoral shaft in the longitudinal and transverse directions. Higher values for reduced modulus are obtained for indentations in the longitudinal direction, with anisotropy ratios of 1.72 ± 0.40 (P), 1.75 ± 0.69 (C) and 1.34 ± 0.30 (D). Hardness is also higher in the longitudinal direction, with anisotropic ratios of 1.35 ± 0.27 (P), 1.35 ± 0.47 (C) and 1.17 ± 0.19 (D). We observed a significant anisotropy in the micromechanical properties of the mouse femur, but the correlation for reduced modulus and hardness between the two directions is low ( < 0.3) and not significant. Therefore, we highly recommend performing independent indentation testing in both the longitudinal and transverse directions when knowledge of the tissue mechanical behaviour along multiple directions is required.

Citing Articles

Hormonal and non-hormonal oral contraceptives given long-term to pubertal rats differently affect bone mass, quality and metabolism.

Porwal K, Sharma S, Kumar S, Tomar M, Sadhukhan S, Rajput S Front Endocrinol (Lausanne). 2023; 14:1233613.

PMID: 37664835 PMC: 10470083. DOI: 10.3389/fendo.2023.1233613.

Variation in Juvenile Long Bone Properties as a Function of Age: Mechanical and Compositional Characterization.

Vazquez Sanz C, Victoria Rodriguez I, Forriol F, Tejado E, Lopez-Valdes F Materials (Basel). 2023; 16(4).

PMID: 36837267 PMC: 9967109. DOI: 10.3390/ma16041637.

Bone matrix quality in a developing high-fat diet mouse model is altered by RAGE deletion.

Stephen S, Bailey S, DErminio D, Krishnamoorthy D, Iatridis J, Vashishth D Bone. 2022; 162:116470.

PMID: 35718325 PMC: 9296598. DOI: 10.1016/j.bone.2022.116470.

Brillouin-Raman microspectroscopy for the morpho-mechanical imaging of human lamellar bone.

Alunni Cardinali M, Di Michele A, Mattarelli M, Caponi S, Govoni M, Dallari D J R Soc Interface. 2022; 19(187):20210642.

PMID: 35104431 PMC: 8807060. DOI: 10.1098/rsif.2021.0642.

LDN Protects Bone Property Deterioration at Different Hierarchical Levels in T2DM Mice Bone.

Shitole P, Choubey A, Mondal P, Ghosh R ACS Omega. 2021; 6(31):20369-20378.

PMID: 34395985 PMC: 8358965. DOI: 10.1021/acsomega.1c02371.

References

Franzoso G, Zysset P . Elastic anisotropy of human cortical bone secondary osteons measured by nanoindentation. J Biomech Eng. 2008; 131(2):021001. DOI: 10.1115/1.3005162. View

Hofmann T, Heyroth F, Meinhard H, Franzel W, Raum K . Assessment of composition and anisotropic elastic properties of secondary osteon lamellae. J Biomech. 2005; 39(12):2282-94. DOI: 10.1016/j.jbiomech.2005.07.009. View

Voide R, Schneider P, Stauber M, Wyss P, Stampanoni M, Sennhauser U . Time-lapsed assessment of microcrack initiation and propagation in murine cortical bone at submicrometer resolution. Bone. 2009; 45(2):164-73. DOI: 10.1016/j.bone.2009.04.248. View

Fantner G, Hassenkam T, Kindt J, Weaver J, Birkedal H, Pechenik L . Sacrificial bonds and hidden length dissipate energy as mineralized fibrils separate during bone fracture. Nat Mater. 2005; 4(8):612-6. DOI: 10.1038/nmat1428. View

de Souza R, Matsuura M, Eckstein F, Rawlinson S, Lanyon L, Pitsillides A . Non-invasive axial loading of mouse tibiae increases cortical bone formation and modifies trabecular organization: a new model to study cortical and cancellous compartments in a single loaded element. Bone. 2005; 37(6):810-8. DOI: 10.1016/j.bone.2005.07.022. View

OBrien F, Hardiman D, Hazenberg J, Mercy M, Mohsin S, Taylor D . The behaviour of microcracks in compact bone. Eur J Morphol. 2005; 42(1-2):71-9. DOI: 10.1080/09243860500096131. View

Carnelli D, Vena P, Dao M, Ortiz C, Contro R . Orientation and size-dependent mechanical modulation within individual secondary osteons in cortical bone tissue. J R Soc Interface. 2013; 10(81):20120953. PMC: 3627101. DOI: 10.1098/rsif.2012.0953. View

Rho J, Roy 2nd M, Tsui T, Pharr G . Elastic properties of microstructural components of human bone tissue as measured by nanoindentation. J Biomed Mater Res. 1999; 45(1):48-54. DOI: 10.1002/(sici)1097-4636(199904)45:1<48::aid-jbm7>3.0.co;2-5. View

Fan Z, Rho J . Effects of viscoelasticity and time-dependent plasticity on nanoindentation measurements of human cortical bone. J Biomed Mater Res A. 2003; 67(1):208-14. DOI: 10.1002/jbm.a.10027. View

10.

Rodriguez-Florez N, Oyen M, Shefelbine S . Insight into differences in nanoindentation properties of bone. J Mech Behav Biomed Mater. 2012; 18:90-9. DOI: 10.1016/j.jmbbm.2012.11.005. View

11.

Leong P, Morgan E . Correlations between indentation modulus and mineral density in bone-fracture calluses. Integr Comp Biol. 2011; 49(1):59-68. PMC: 3202910. DOI: 10.1093/icb/icp024. View

12.

Akhter M, Fan Z, Rho J . Bone intrinsic material properties in three inbred mouse strains. Calcif Tissue Int. 2004; 75(5):416-20. DOI: 10.1007/s00223-004-0241-7. View

13.

Casanova M, Balmelli A, Carnelli D, Courty D, Schneider P, Muller R . Nanoindentation analysis of the micromechanical anisotropy in mouse cortical bone. R Soc Open Sci. 2017; 4(2):160971. PMC: 5367284. DOI: 10.1098/rsos.160971. View

14.

Silva M, Brodt M, Fan Z, Rho J . Nanoindentation and whole-bone bending estimates of material properties in bones from the senescence accelerated mouse SAMP6. J Biomech. 2004; 37(11):1639-46. DOI: 10.1016/j.jbiomech.2004.02.018. View

15.

Lipson S, Katz J . The relationship between elastic properties and microstructure of bovine cortical bone. J Biomech. 1984; 17(4):231-40. DOI: 10.1016/0021-9290(84)90134-9. View

16.

Swadener J, Rho J, Pharr G . Effects of anisotropy on elastic moduli measured by nanoindentation in human tibial cortical bone. J Biomed Mater Res. 2001; 57(1):108-12. DOI: 10.1002/1097-4636(200110)57:1<108::aid-jbm1148>3.0.co;2-6. View

17.

Dong X, Guo X . The dependence of transversely isotropic elasticity of human femoral cortical bone on porosity. J Biomech. 2004; 37(8):1281-7. DOI: 10.1016/j.jbiomech.2003.12.011. View

18.

Carnelli D, Lucchini R, Ponzoni M, Contro R, Vena P . Nanoindentation testing and finite element simulations of cortical bone allowing for anisotropic elastic and inelastic mechanical response. J Biomech. 2011; 44(10):1852-8. DOI: 10.1016/j.jbiomech.2011.04.020. View

19.

Rho J, Pharr G . Effects of drying on the mechanical properties of bovine femur measured by nanoindentation. J Mater Sci Mater Med. 2004; 10(8):485-8. DOI: 10.1023/a:1008901109705. View

20.

Vashishth D, Tanner K, Bonfield W . Fatigue of cortical bone under combined axial-torsional loading. J Orthop Res. 2001; 19(3):414-20. DOI: 10.1016/S0736-0266(00)00036-X. View