Development of an Improved Standard Reference Material for Vitamin D Metabolites in Human Serum

Overview

Journal Anal Chem

Specialty Chemistry

Date 2017 Apr 5

PMID 28375002

Citations 20

Authors

Karen W Phinney

Susan S-C Tai

Mary Bedner

Johanna E Camara

Rosalind R C Chia

Lane C Sander

Katherine E Sharpless

Stephen A Wise

James H Yen

Rosemary L Schleicher

Madhulika Chaudhary-Webb

Khin L Maw

Yasamin Rahmani

Joseph M Betz

Joyce Merkel

Christopher T Sempos

Paul M Coates

Ramon A Durazo-Arvizu

Kurtis Sarafin

Stephen P J Brooks

Affiliations

Soon will be listed here.

Abstract

The National Institute of Standards and Technology (NIST) has developed Standard Reference Material (SRM) 972a Vitamin D Metabolites in Frozen Human Serum as a replacement for SRM 972, which is no longer available. SRM 972a was developed in collaboration with the National Institutes of Health's Office of Dietary Supplements. In contrast to the previous reference material, three of the four levels of SRM 972a are composed of unmodified human serum. This SRM has certified and reference values for the following 25-hydroxyvitamin D [25(OH)D] species: 25(OH)D, 25(OH)D, and 3-epi-25(OH)D. The value assignment and certification process included three isotope-dilution mass spectrometry approaches, with measurements performed at NIST and at the Centers for Disease Control and Prevention (CDC). The value assignment methods employed have been modified from those utilized for the previous SRM, and all three approaches now incorporate chromatographic resolution of the stereoisomers, 25(OH)D and 3-epi-25(OH)D.

Citing Articles

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Wise S, Cavalier E, Lukas P, Peeters S, Le Goff C, Briggs L Anal Bioanal Chem. 2025; .

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Evolution and impact of Standard Reference Materials (SRMs) for determining vitamin D metabolites.

Wise S, Kuszak A, Camara J Anal Bioanal Chem. 2024; 416(9):2335-2358.

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Multicenter comparison of analytical interferences of 25-OH vitamin D immunoassay and mass spectrometry methods by endogenous interferents and cross-reactivity with 3-epi-25-OH-vitamin D.

Lee J, Seo J, Lee K, Roh E, Yun Y, Lee Y Pract Lab Med. 2024; 38:e00347.

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Determination of 24,25-dihydroxyvitamin D in Vitamin D External Quality Assessment Scheme samples using a reference measurement procedure.

Wise S, Hahm G, Burdette C, Tai S, Camara J, Sempos C J Steroid Biochem Mol Biol. 2023; 231:106318.

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