Development and Certification of a Standard Reference Material for Vitamin D Metabolites in Human Serum

Overview

Journal Anal Chem

Specialty Chemistry

Date 2011 Dec 7

PMID 22141317

Citations 60

Authors

Karen W Phinney

Mary Bedner

Susan S-C Tai

Veronica V Vamathevan

Lane C Sander

Katherine E Sharpless

Stephen A Wise

James H Yen

Rosemary L Schleicher

Madhulika Chaudhary-Webb

Christine M Pfeiffer

Joseph M Betz

Paul M Coates

Mary Frances Picciano

Affiliations

Soon will be listed here.

Abstract

The National Institute of Standards and Technology (NIST), in collaboration with the National Institutes of Health's Office of Dietary Supplements (NIH-ODS), has developed a Standard Reference Material (SRM) for the determination of 25-hydroxyvitamin D [25(OH)D] in serum. SRM 972 Vitamin D in Human Serum consists of four serum pools with different levels of vitamin D metabolites and has certified and reference values for 25(OH)D(2), 25(OH)D(3), and 3-epi-25(OH)D(3). Value assignment of this SRM was accomplished using a combination of three isotope-dilution mass spectrometry approaches, with measurements performed at NIST and at the Centers for Disease Control and Prevention (CDC). Chromatographic resolution of the 3-epimer of 25(OH)D(3) proved to be essential for accurate determination of the metabolites.

Citing Articles

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