Infections of Virulent and Avirulent Viruses Differentially Influenced the Expression of Dicer-1, Ago-1, and MicroRNAs in Bombus Terrestris

Overview

Journal Sci Rep

Specialty Science

Date 2017 Apr 5

PMID 28374846

Citations 6

Authors

Jinzhi Niu

Ivan Meeus

Dieter Im De Coninck

Dieter Deforce

Kayvan Etebari

Sassan Asgari

Guy Smagghe

Affiliations

Soon will be listed here.

Abstract

The microRNA (miRNA) pathway is well established to be involved in host-pathogen interactions. As key insect pollinators, bees are suffering from widely spreading viruses, especially honeybees and bumblebees. In order to better understand bee-virus interaction, we comparatively analyzed the involvement of the bumblebee miRNA pathway upon infection by two different viruses. In our setup, an avirulent infection is induced by slow bee paralysis virus (SBPV) and a virulent infection is induced by Israeli acute paralysis virus (IAPV). Our results showed the increased expressions of dicer-1 and ago-1 upon SBPV infection. There were 17 and 12 bumblebee miRNAs differentially expressed upon SBPV and IAPV infections, respectively. These results may indicate the involvement of the host miRNA pathway in bumblebee-virus interaction. However, silencing of dicer-1 did not influence the genome copy number of SBPV. Target prediction for these differentially expressed miRNAs showed their possible involvement in targeting viral genomic RNA and in the regulation of networks in bumblebee. Our study opens a new insight into bee-virus interaction meditated by host miRNAs.

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