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Construction of a Yeast Mutant Lacking the Mitochondrial Nuclease

Overview
Journal Nucleic Acids Res
Publisher Oxford University Press
Specialty Biochemistry
Date 1988 Apr 25
PMID 2836791
Citations 32
Authors
H P Zassenhaus
T J Hofmann
R Uthayashanker
R D Vincent
M Zona
Affiliations
Soon will be listed here.
Abstract

The nuclear gene from Saccharomyces cerevisiae that encodes the major mitochondrial nuclease was cloned. Gene sequences were identified from a lambda gt11 library by antibodies specific to the mitochondrial nuclease. DNA from the phage recombinant was used to isolate the entire nuclease gene from a plasmid library. Yeast strains containing the nuclease gene on a multicopy plasmid vector overproduced mitochondrial nuclease 20-40 times relative to a wild-type strain. Strains containing a null allele of the nuclease gene lacked all traces of mitochondrial nuclease. Both cell types, however, were phenotypically wild-type indicating that the nuclease is not an essential enzyme for mitochondrial function. The locus encoding the mitochondrial nuclease is termed NUC1.

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