A Vesicle Trafficking Protein αSNAP Regulates Paneth Cell Differentiation In vivo

Overview

Journal Biochem Biophys Res Commun

Publisher Elsevier

Specialty Biochemistry

Date 2017 Apr 1

PMID 28359759

Citations 1

Authors

Susana Lechuga

Nayden G Naydenov

Alex Feygin

Antonio J Jimenez

Andrei I Ivanov

Affiliations

Soon will be listed here.

Abstract

A soluble N-ethylmaleimide-sensitive factor-attachment protein alpha (αSNAP) is a multifunctional scaffolding protein that regulates intracellular vesicle trafficking and signaling. In cultured intestinal epithelial cells, αSNAP has been shown to be essential for cell survival, motility, and adhesion; however, its physiologic functions in the intestinal mucosa remain unknown. In the present study, we used a mouse with a spontaneous hydrocephalus with hop gait (hyh) mutation of αSNAP to examine the roles of this trafficking protein in regulating intestinal epithelial homeostasis in vivo. Homozygous hyh mice demonstrated decreased expression of αSNAP protein in the intestinal epithelium, but did not display gross abnormalities of epithelial architecture in the colon and ileum. Such αSNAP depletion attenuated differentiation of small intestinal epithelial enteroids ex vivo. Furthermore, αSNAP-deficient mutant animals displayed reduced formation of lysozyme granules in small intestinal crypts and decreased expression of lysozyme and defensins in the intestinal mucosa, which is indicative of defects in Paneth cell differentiation. By contrast, development of Goblet cells, enteroendocrine cells, and assembly of enterocyte apical junctions was not altered in hyh mutant mice. Our data revealed a novel role of αSNAP in the intestinal Paneth cell differentiation in vivo.

Citing Articles

Loss of β-Cytoplasmic Actin in the Intestinal Epithelium Increases Gut Barrier Permeability and Exaggerates the Severity of Experimental Colitis.

Lechuga S, Naydenov N, Feygin A, Cruise M, Ervasti J, Ivanov A Front Cell Dev Biol. 2020; 8:588836.

PMID: 33195251 PMC: 7644907. DOI: 10.3389/fcell.2020.588836.

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