Separation of Simian Virus 40 Large-T-antigen-transforming and Origin-binding Functions from the Ability to Block Differentiation

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1988 Mar 1

PMID 2835674

Citations 24

Authors

V Cherington

M Brown

E Paucha

J St Louis

B M Spiegelman

T M Roberts

Affiliations

Soon will be listed here.

Abstract

Wild-type simian virus 40 large T antigen is very effective at blocking adipocyte differentiation in 3T3-F442A cells as assayed by triglyceride accumulation, induction of glycerophosphate dehydrogenase activity, and expression of mRNAs for glycerophosphate dehydrogenase, the adipocyte serine protease adipsin, and the putative lipid-binding protein adipocyte P2. Point mutants defective for either origin-specific DNA binding or transformation blocked differentiation as completely as wild type.

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