Effects of Storage Time on Glycolysis in Donated Human Blood Units

Overview

Journal Metabolites

Publisher MDPI

Date 2017 Mar 30

PMID 28353627

Citations 3

Authors

Zhen Qi

John D Roback

Eberhard O Voit

Affiliations

Soon will be listed here.

Abstract

: Donated blood is typically stored before transfusions. During storage, the metabolism of red blood cells changes, possibly causing storage lesions. The changes are storage time dependent and exhibit donor-specific variations. It is necessary to uncover and characterize the responsible molecular mechanisms accounting for such biochemical changes, qualitatively and quantitatively; : Based on the integration of metabolic time series data, kinetic models, and a stoichiometric model of the glycolytic pathway, a customized inference method was developed and used to quantify the dynamic changes in glycolytic fluxes during the storage of donated blood units. The method provides a proof of principle for the feasibility of inferences regarding flux characteristics from metabolomics data; : Several glycolytic reaction steps change substantially during storage time and vary among different fluxes and donors. The quantification of these storage time effects, which are possibly irreversible, allows for predictions of the transfusion outcome of individual blood units; : The improved mechanistic understanding of blood storage, obtained from this computational study, may aid the identification of blood units that age quickly or more slowly during storage, and may ultimately improve transfusion management in clinics.

Citing Articles

An Insight into the Stages of Ion Leakage during Red Blood Cell Storage.

Zimna A, Kaczmarska M, Szczesny-Malysiak E, Wajda A, Bulat K, Alcicek F Int J Mol Sci. 2021; 22(6).

PMID: 33809183 PMC: 7998123. DOI: 10.3390/ijms22062885.

Restoration of Physiological Levels of Uric Acid and Ascorbic Acid Reroutes the Metabolism of Stored Red Blood Cells.

Bardyn M, Chen J, Dussiot M, Crettaz D, Schmid L, Langst E Metabolites. 2020; 10(6).

PMID: 32486030 PMC: 7344535. DOI: 10.3390/metabo10060226.

Red blood cell storage lesion: causes and potential clinical consequences.

Yoshida T, Prudent M, DAlessandro A Blood Transfus. 2019; 17(1):27-52.

PMID: 30653459 PMC: 6343598. DOI: 10.2450/2019.0217-18.

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