Characterization of a Chloroplast Mutation in the PsaA2 Gene of Chlamydomonas Reinhardtii

Overview

Journal Curr Genet

Specialty Genetics

Date 1987 Jan 1

PMID 2834098

Citations 26

Authors

J Girard-Bascou

Y Choquet

M Schneider

M Delosme

M Dron

Affiliations

Soon will be listed here.

Abstract

The synthesis of polypeptides related to the CPI chlorophyll-protein complex of photosystem I has been studied by pulse-labeling experiments in twenty chloroplast mutants of Chlamydomonas reinhardtii. Three mutations of the same locus (Girard-Bascou 1987) result in the absence of these CPI-related polypeptides. Among these mutations one, (FUD26) leads to the synthesis of a new polypeptide presumed to be a truncated CPI apoprotein. The molecular characterization of this mutation in the psaA2 gene has been achieved by DNA sequencing the 3' end of this gene. The FUD26 mutation is a 4 base pair deletion resulting in frameshift and premature termination of the protein.

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