Genetic Engineering of Thylakoid Protein Complexes by Chloroplast Transformation in Chlamydomonas Reinhardtii

Overview

Journal Photosynth Res

Publisher Springer

Date 2013 Dec 6

PMID 24307038

Citations 1

Authors

A N Webber

S E Bingham

H Lee

Affiliations

Soon will be listed here.

Abstract

Chloroplast transformation of Chlamydomonas reinhardtii has developed into a powerful tool for studying the structure, function and assembly of thylakoid protein complexes in a eukaryotic organism. In this article we review the progress that is being made in the development of procedures for efficient chloroplast transformation. This focuses on the development of selectable markers and the use of Chlamydomonas mutants, individually lacking thylakoid protein complexes, as recipients. Chloroplast transformation has now been used to engineer all four major thylakoid protein complexes, photosystem II, photosystem I, cytochrome b 6/f and ATP synthase. These results are discussed with an emphasis on new insights into assembly and function of these complexes in chloroplasts as compared with their prokaryotic counterparts.

Citing Articles

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Yu Z, Vermaas W J Bacteriol. 2000; 183(1):145-54.

PMID: 11114911 PMC: 94860. DOI: 10.1128/JB.183.1.145-154.2001.

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