Bioengineering Approaches to Mature Human Pluripotent Stem Cell-Derived Cardiomyocytes

Overview

Journal Front Cell Dev Biol

Specialty Cell Biology

Date 2017 Mar 25

PMID 28337437

Citations 20

Authors

Xuetao Sun

Sara S Nunes

Affiliations

Soon will be listed here.

Abstract

Human pluripotent stem cell-derived cardiomyocytes (hPSC-CM) represent a potential unlimited cell supply for cardiac tissue engineering and possibly regenerative medicine applications. However, hPSC-CMs produced by current protocols are not representative of native adult human cardiomyocytes as they display immature gene expression profile, structure and function. In order to improve hPSC-CM maturity and function, various approaches have been developed, including genetic manipulations to induce gene expression, delivery of biochemical factors, such as triiodothyronine and alpha-adrenergic agonist phenylephrine, induction of cell alignment in 3D tissues, mechanical stress as a mimic of cardiac load and electrical stimulation/pacing or a combination of these. In this mini review, we discuss biomimetic strategies for the maturation for hPSC-CMs with a particular focus on electromechanical conditioning methods.

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