Proviral-activated C-erbB is Leukemogenic but Not Sarcomagenic: Characterization of a Replication-competent Retrovirus Containing the Activated C-erbB

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1988 May 1

PMID 2833627

Citations 17

Authors

R J Pelley

C MOSCOVICI

S Hughes

H J Kung

Affiliations

Soon will be listed here.

Abstract

Avian leukosis virus (ALV) induces erythroblastosis in chickens by integrating its DNA into the host c-erbB locus and by activating expression of truncated c-erbB transcripts. Although there is a 100% correlation of c-erbB activation with ALV-induced erythroblastosis, direct evidence that the activated c-erbB is oncogenic has not been established. We have constructed a replication-competent retrovirus containing the activated c-erbB to investigate its transforming potential. The Rous c-erbB virus (REB-c) was constructed by inserting the activated c-erbB cDNA into a Rous sarcoma virus vector in place of src. When transfected into transformed quail fibroblasts (QT6), the REB-c construct stably integrates and expresses c-erbB-specific transcripts and produces infectious virus. The REB-c retrovirus produces short-latency polyclonal erythroblastosis in chickens. However, in contrast to avian erythroblastosis virus which contains v-erbB, the REB-c construct does not transform chicken embryo fibroblasts in vitro, nor does the REB-c virus produce sarcomas when injected into the wing web of chickens. Our results provide the first direct evidence that the activated c-erbB which lacks the amino-terminal extracellular domain but which retains the entire carboxy-terminal sequences is leukemogenic but not sarcomagenic.

Citing Articles

EGF receptor in neoplasia and metastasis.

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Induction of renal adenocarcinoma by a nonmutated erbB oncogene.

Banner B, Davis R, Robinson H J Virol. 1993; 67(2):1132-6.

PMID: 8093489 PMC: 237473. DOI: 10.1128/JVI.67.2.1132-1136.1993.

Modulation of erbB kinase activity and oncogenic potential by single point mutations in the glycine loop of the catalytic domain.

Shu H, Chang C, Ravi L, Ling L, Castellano C, Walter E Mol Cell Biol. 1994; 14(10):6868-78.

PMID: 7935404 PMC: 359217. DOI: 10.1128/mcb.14.10.6868-6878.1994.

Tyrosine kinase activity may be necessary but is not sufficient for c-erbB1-mediated tissue-specific tumorigenicity.

Connolly D, Toutenhoofd S, Maihle N J Virol. 1994; 68(10):6804-10.

PMID: 7916062 PMC: 237108. DOI: 10.1128/JVI.68.10.6804-6810.1994.

A minor tyrosine phosphorylation site located within the CAIN domain plays a critical role in regulating tissue-specific transformation by erbB kinase.

Chang C, Shu H, Ravi L, Pelley R, Shu H, Kung H J Virol. 1995; 69(2):1172-80.

PMID: 7815495 PMC: 188690. DOI: 10.1128/JVI.69.2.1172-1180.1995.

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