A Wnt5 Activity Asymmetry and Intercellular Signaling Via PCP Proteins Polarize Node Cells for Left-Right Symmetry Breaking

Overview

Journal Dev Cell

Publisher Cell Press

Specialties Cell Biology
Reproductive Medicine

Date 2017 Mar 16

PMID 28292423

Citations 56

Authors

Katsura Minegishi

Masakazu Hashimoto

Rieko Ajima

Katsuyoshi Takaoka

Kyosuke Shinohara

Yayoi Ikawa

Hiromi Nishimura

Andrew P McMahon

Karl Willert

Yasushi Okada

Hiroshi Sasaki

Dongbo Shi

Toshihiko Fujimori

Toshihisa Ohtsuka

Yasunobu Igarashi

Terry P Yamaguchi

Akihiko Shimono

Hidetaka Shiratori

Hiroshi Hamada

Affiliations

Soon will be listed here.

Abstract

Polarization of node cells along the anterior-posterior axis of mouse embryos is responsible for left-right symmetry breaking. How node cells become polarized has remained unknown, however. Wnt5a and Wnt5b are expressed posteriorly relative to the node, whereas genes for Sfrp inhibitors of Wnt signaling are expressed anteriorly. Here we show that polarization of node cells is impaired in Wnt5aWnt5b and Sfrp mutant embryos, and also in the presence of a uniform distribution of Wnt5a or Sfrp1, suggesting that Wnt5 and Sfrp proteins act as instructive signals in this process. The absence of planar cell polarity (PCP) core proteins Prickle1 and Prickle2 in individual cells or local forced expression of Wnt5a perturbed polarization of neighboring wild-type cells. Our results suggest that opposing gradients of Wnt5a and Wnt5b and of their Sfrp inhibitors, together with intercellular signaling via PCP proteins, polarize node cells along the anterior-posterior axis for breaking of left-right symmetry.

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