Sensitivity to Oxazolone Induced Dermatitis is Transferable with Gut Microbiota in Mice

Overview

Journal Sci Rep

Specialty Science

Date 2017 Mar 15

PMID 28290517

Citations 34

Authors

Line Fisker Zachariassen

Lukasz Krych

Kare Engkilde

Dennis Sandris Nielsen

Witold Kot

Camilla Hartmann Friis Hansen

Axel Kornerup Hansen

Affiliations

Soon will be listed here.

Abstract

Atopic Dermatitis (AD) has been associated with gut microbiota (GM) dysbiosis in humans, indicating a causative role of GM in AD etiology. Furthermore, the GM strongly correlates to essential disease parameters in the well-known oxazolone-induced mouse model of AD. Here, we demonstrate that it is possible to transfer both a high-responding and a low-responding AD phenotype with GM from conventional mice to germ-free mice. The mice inoculated with the high-responding GM had significantly higher clinical score, increased ear thickness, and increased levels of IL-1β, TNFα, IL-4, IL-5, and IL-6 compared to the mice inoculated with the low-responding GM. The inter-individual variation was in general not affected by this increase in effect size. Germ-free mice induced with AD revealed a high disease response as well as high inter-individual variation indicating protective properties of certain microbial taxa in this model. This study underlines that the GM has a strong impact on AD in mouse models, and that the power of studies may be increased by the application of mice inoculated with a specific GM from high responders to increase the effect size.

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