Systematic and Functional Identification of Small Non-coding RNAs Associated with Exogenous Biofuel Stress in Cyanobacterium Sp. PCC 6803

Overview

Journal Biotechnol Biofuels

Publisher Biomed Central

Specialty Biotechnology

Date 2017 Mar 14

PMID 28286552

Citations 11

Authors

Guangsheng Pei

Tao Sun

Shuo Chen

Lei Chen

Weiwen Zhang

Affiliations

Soon will be listed here.

Abstract

Background: The unicellular model cyanobacterium sp. PCC 6803 is considered a promising microbial chassis for biofuel production. However, its low tolerance to biofuel toxicity limits its potential application. Although recent studies showed that bacterial small RNAs (sRNAs) play important roles in regulating cellular processes in response to various stresses, the role of sRNAs in resisting exogenous biofuels is yet to be determined.

Results: Based on genome-wide sRNA sequencing combined with systematic analysis of previous transcriptomic and proteomic data under the same biofuel or environmental perturbations, we report the identification of 133 -encoded sRNA transcripts with high-resolution mapping of sRNAs in , including 23 novel sRNAs identified for the first time. In addition, according to quantitative expression analysis and sRNA regulatory network prediction, sRNAs potentially involved in biofuel tolerance were identified and functionally confirmed by constructing sRNA overexpression or suppression strains of . Notably, overexpression of sRNA Nc117 revealed an improved tolerance to ethanol and butanol, while suppression of Nc117 led to increased sensitivity.

Conclusions: The study provided the first comprehensive responses to exogenous biofuels at the sRNA level in and opens an avenue to engineering sRNA regulatory elements for improved biofuel tolerance in the cyanobacterium s.

Citing Articles

Review of recent advances in improvement strategies for biofuels production from cyanobacteria.

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Proteomic Responses to Butanol Stress.

Costa P, Usai G, Re A, Manfredi M, Mannino G, Bertea C Front Microbiol. 2021; 12:674639.

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Genetic, Genomics, and Responses to Stresses in Cyanobacteria: Biotechnological Implications.

Cassier-Chauvat C, Blanc-Garin V, Chauvat F Genes (Basel). 2021; 12(4).

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Impact of RNase E and RNase J on Global mRNA Metabolism in the Cyanobacterium PCC6803.

Cavaiuolo M, Chagneau C, Laalami S, Putzer H Front Microbiol. 2020; 11:1055.

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The Small RNA sr8384 Is a Crucial Regulator of Cell Growth in Solventogenic Clostridia.

Yang Y, Zhang H, Lang N, Zhang L, Chai C, He H Appl Environ Microbiol. 2020; 86(13).

PMID: 32358006 PMC: 7301862. DOI: 10.1128/AEM.00665-20.

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