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Blood DNA Methylation Pattern is Altered in Mesial Temporal Lobe Epilepsy

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Journal Sci Rep
Specialty Science
Date 2017 Mar 10
PMID 28276448
Citations 21
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Abstract

Mesial temporal lobe epilepsy (MTLE) is a common epileptic disorder; little is known whether it is associated with peripheral epigenetic changes. Here we compared blood whole genomic DNA methylation pattern in MTLE patients (n = 30) relative to controls (n = 30) with the Human Methylation 450 K BeadChip assay, and explored genes and pathways that were differentially methylated using bioinformatics profiling. The MTLE and control groups showed significantly different (P < 1.03e-07) DNA methylation at 216 sites, with 164 sites involved hyper- and 52 sites hypo- methylation. Two hyper- and 32 hypo-methylated sites were associated with promoters, while 87 hyper- and 43 hypo-methylated sites corresponded to coding regions. The differentially methylated genes were largely related to pathways predicted to participate in anion binding, oxidoreductant activity, growth regulation, skeletal development and drug metabolism, with the most distinct ones included SLC34A2, CLCN6, CLCA4, CYP3A43, CYP3A4 and CYP2C9. Among the MTLE patients, panels of genes also appeared to be differentially methylated relative to disease duration, resistance to anti-epileptics and MRI alterations of hippocampal sclerosis. The peripheral epigenetic changes observed in MTLE could be involved in certain disease-related modulations and warrant further translational investigations.

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References
1.
Guengerich F . Cytochrome p450 and chemical toxicology. Chem Res Toxicol. 2007; 21(1):70-83. DOI: 10.1021/tx700079z. View

2.
Pirker S, Gasser E, Czech T, Baumgartner C, Schuh E, Feucht M . Dynamic up-regulation of prodynorphin transcription in temporal lobe epilepsy. Hippocampus. 2009; 19(11):1051-4. PMC: 3073604. DOI: 10.1002/hipo.20633. View

3.
He S, Wang Q, He J, Pu H, Yang W, Ji J . Proteomic analysis and comparison of the biopsy and autopsy specimen of human brain temporal lobe. Proteomics. 2006; 6(18):4987-96. DOI: 10.1002/pmic.200600078. View

4.
Bedner P, Dupper A, Huttmann K, Muller J, Herde M, Dublin P . Astrocyte uncoupling as a cause of human temporal lobe epilepsy. Brain. 2015; 138(Pt 5):1208-22. PMC: 5963418. DOI: 10.1093/brain/awv067. View

5.
Li A, Choi Y, Dziema H, Cao R, Cho H, Jung Y . Proteomic profiling of the epileptic dentate gyrus. Brain Pathol. 2010; 20(6):1077-89. PMC: 2951482. DOI: 10.1111/j.1750-3639.2010.00414.x. View