NMR-based Stable Isotope Resolved Metabolomics in Systems Biochemistry

Overview

Journal Arch Biochem Biophys

Publisher Elsevier

Specialties Biochemistry
Biophysics

Date 2017 Mar 7

PMID 28263717

Citations 24

Authors

Andrew N Lane

Teresa W-M Fan

Affiliations

Soon will be listed here.

Abstract

Metabolism is the basic activity of live cells, and monitoring the metabolic state provides a dynamic picture of the cells or tissues, and how they respond to external changes, for in disease or treatment with drugs. NMR is an extremely versatile analytical tool that can be applied to a wide range of biochemical problems. Despite its modest sensitivity its versatility make it an ideal tool for analyzing biochemical dynamics both in vitro and in vivo, especially when coupled with its isotope editing capabilities, from which isotope distributions can be readily determined. These are critical for any analyses of flux in live organisms. This review focuses on the utility of NMR spectroscopy in metabolomics, with an emphasis on NMR applications in stable isotope-enriched tracer research for elucidating biochemical pathways and networks with examples from nucleotide biochemistry. The knowledge gained from this area of research provides a ready link to genomic, epigenomic, transcriptomic, and proteomic information to achieve systems biochemical understanding of living cells and organisms.

Citing Articles

NMR-Based Stable Isotope Tracing of Cancer Metabolism.

Lin P, Lane A, W-M Fan T Methods Mol Biol. 2024; 2855:457-504.

PMID: 39354323 DOI: 10.1007/978-1-0716-4116-3_26.

Stable isotope-resolved metabolomics analyses of metabolic phenotypes reveal variable glutamine metabolism in different patient-derived models of non-small cell lung cancer from a single patient.

Kinslow C, Ll M, Cai Y, Yan J, Lorkiewicz P, Al-Attar A Metabolomics. 2024; 20(4):87.

PMID: 39068202 PMC: 11317205. DOI: 10.1007/s11306-024-02126-x.

Challenges of Spatially Resolved Metabolism in Cancer Research.

Lane A, Higashi R, W-M Fan T Metabolites. 2024; 14(7).

PMID: 39057706 PMC: 11278851. DOI: 10.3390/metabo14070383.

The Role of Amino Acids in Non-Enzymatic Antioxidant Mechanisms in Cancer: A Review.

Dyachenko E, Belskaya L Metabolites. 2024; 14(1).

PMID: 38248831 PMC: 10818545. DOI: 10.3390/metabo14010028.

Metabolomics: A New Era in the Diagnosis or Prognosis of B-Cell Non-Hodgkin's Lymphoma.

Alfaifi A, Refai M, Alsaadi M, Bahashwan S, Malhan H, Al-Kahiry W Diagnostics (Basel). 2023; 13(5).

PMID: 36900005 PMC: 10000528. DOI: 10.3390/diagnostics13050861.

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