A Single Base-pair Change Creates a Chi Recombinational Hotspot in Bacteriophage Lambda

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1978 Dec 1

PMID 282634

Citations 15

Authors

K U Sprague

D H Faulds

G R Smith

Affiliations

Soon will be listed here.

Abstract

X4+ mutations, responsible for the Chi phenotype in phage lambda, locally increase the rate of recombination promoted by the Escherichia coli recombination system (Rec). X+ mutations in the cII gene, one of a few sites in lambda at which such mutations arise, were located genetically and physically with overlapping deletions. DNA sequence analysis of the deletion segment containing the X+ C mutations showed that two independent X+ C mutations arose by the same A-T to T-A transversion. Presumably, this change creates a nucleotide sequence recognized by a protein involved in a rate-limiting step of recombination.

Citing Articles

RecBCD enzyme and Chi recombination hotspots as determinants of self vs. non-self: Myths and mechanisms.

Subramaniam S, Smith G Adv Genet. 2022; 109:1-37.

PMID: 36334915 PMC: 10047805. DOI: 10.1016/bs.adgen.2022.06.001.

Akaby-Cell-free protein expression system for linear templates.

Sato W, Sharon J, Deich C, Gaut N, Cash B, Engelhart A PLoS One. 2022; 17(4):e0266272.

PMID: 35390057 PMC: 8989226. DOI: 10.1371/journal.pone.0266272.

RecBCD Enzyme "Chi Recognition" Mutants Recognize Chi Recombination Hotspots in the Right DNA Context.

Amundsen S, Sharp J, Smith G Genetics. 2016; 204(1):139-52.

PMID: 27401752 PMC: 5012381. DOI: 10.1534/genetics.116.191056.

RecBCD enzyme and the repair of double-stranded DNA breaks.

Dillingham M, Kowalczykowski S Microbiol Mol Biol Rev. 2008; 72(4):642-71, Table of Contents.

PMID: 19052323 PMC: 2593567. DOI: 10.1128/MMBR.00020-08.

Gene replacement with linear DNA fragments in wild-type Escherichia coli: enhancement by Chi sites.

Dabert P, Smith G Genetics. 1997; 145(4):877-89.

PMID: 9093843 PMC: 1207893. DOI: 10.1093/genetics/145.4.877.

References

Rosenberg M, Court D, Shimatake H, Brady C, WULFF D . The relationship between function and DNA sequence in an intercistronic regulatory region in phage lambda. Nature. 1978; 272(5652):414-23. DOI: 10.1038/272414a0. View

Chessin H, Summers W . Initiation by RNA polymerase on UV or x-ray damaged T7 DNA. Biochem Biophys Res Commun. 1970; 38(1):40-5. DOI: 10.1016/0006-291x(70)91080-6. View

Malone R, Chattoraj D, Faulds D, Stahl M, Stahl F . Hotspots for generalized recombination in the Escherichia coli chromosome. J Mol Biol. 1978; 121(4):473-91. DOI: 10.1016/0022-2836(78)90395-9. View

Stahl F, Stahl M . Recombination pathway specificity of Chi. Genetics. 1977; 86(4):715-25. PMC: 1213706. DOI: 10.1093/genetics/86.4.715. View

Maxam A, Gilbert W . A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977; 74(2):560-4. PMC: 392330. DOI: 10.1073/pnas.74.2.560. View

Schwarz E, Scherer G, HOBOM G, Kossel H . Nucleotide sequence of cro, cII and part of the O gene in phage lambda DNA. Nature. 1978; 272(5652):410-4. DOI: 10.1038/272410a0. View

ROBINSON L, Landy A . HindII, HindIII, and HpaI restriction fragment maps of the left arm of bacteriophage lambda DNA. Gene. 1977; 2(1):33-54. DOI: 10.1016/0378-1119(77)90020-8. View

Furth M, Blattner F, McLeester C, Dove W . Genetic structure of the replication origin of bacteriophage lambda. Science. 1977; 198(4321):1046-51. DOI: 10.1126/science.929186. View

Galibert F, Sedat J, Ziff E . Direct determination of DNA nucleotide sequences: structure of a fragment of bacteriophage phiX172 DNA. J Mol Biol. 1974; 87(3):377-407. DOI: 10.1016/0022-2836(74)90093-x. View

10.

Sharp P, Sugden B, Sambrook J . Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry. 1973; 12(16):3055-63. DOI: 10.1021/bi00740a018. View

11.

SIBATANI A . Precipitation and counting of minute quantities of labeled nucleic acids as cetyltrimethylammonium salt. Anal Biochem. 1970; 33(2):279-85. DOI: 10.1016/0003-2697(70)90298-8. View

12.

Smith G . Deletion mutations of the immunity region of coliphage lambda. Virology. 1975; 64(2):544-52. DOI: 10.1016/0042-6822(75)90131-2. View

13.

Henderson D, Weil J . Recombination-deficient deletions in bacteriophage lambda and their interaction with chi mutations. Genetics. 1975; 79(2):143-74. PMC: 1213264. DOI: 10.1093/genetics/79.2.143. View

14.

Belfort M, Noff D, Oppenheim A . Isolation, characterization and deletion mapping of amber mutations in the cll gene of phage lambda. Virology. 1975; 63(1):147-59. DOI: 10.1016/0042-6822(75)90380-3. View

15.

Thomas M, Davis R . Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease. J Mol Biol. 1975; 91(3):315-28. DOI: 10.1016/0022-2836(75)90383-6. View

16.

Murray K, Murray N . Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli. J Mol Biol. 1975; 98(3):551-64. DOI: 10.1016/s0022-2836(75)80086-6. View

17.

Condit R, Steitz J . F factor-mediated inhibition of bacteriophage T7 growth: analysis of T7 RNA and protein synthesis in vivo and in vitro using male and female Escherichia coli. J Mol Biol. 1975; 98(1):31-43. DOI: 10.1016/s0022-2836(75)80099-4. View

18.

Roberts R, Myers P, Morrison A, Murray K . A specific endonuclease from Arthrobacter luteus. J Mol Biol. 1976; 102(1):157-65. DOI: 10.1016/0022-2836(76)90079-6. View

19.

Stahl F, Crasemann J, Stahl M . Rec-mediated recombinational hot spot activity in bacteriophage lambda. III. Chi mutations are site-mutations stimulating rec-mediated recombination. J Mol Biol. 1975; 94(2):203-12. DOI: 10.1016/0022-2836(75)90078-9. View

20.

Berkner K, Folk W . Polynucleotide kinase exchange reaction: quantitave assay for restriction endonuclease-generated 5'-phosphoroyl termini in DNA. J Biol Chem. 1977; 252(10):3176-84. View