Simultaneous Amplification and Identification of 25 Human Papillomavirus Types with Templex Technology

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2006 Sep 29

PMID 17005760

Citations 47

Authors

Jian Han

David C Swan

Sharon J Smith

Shanjuan H Lum

Susan E Sefers

Elizabeth R Unger

Yi-Wei Tang

Affiliations

Soon will be listed here.

Abstract

The majority of existing human papillomavirus (HPV) genotyping assays are based on multiplex PCR using consensus or degenerate primers. We developed a Templex HPV assay that simultaneously detects and identifies 25 common HPV genotypes in a single-tube reaction using type-specific primers for the HPV-specific E6 and E7 genes. The analytical sensitivities of the Templex assay for HPV type 16 (HPV-16), -18, and -56 were 20, 100, and 20 copies per reaction mixture, respectively. The Templex assay provides semiquantitative information on each type when multiple HPV types coexist in one reaction. We tested 109 clinical cervical specimens previously evaluated with the Digene HC2 high-risk HPV DNA test and found 95.4% concordance between the assay results. The Templex assay provided type-specific results and found multiple types in 29.2% (14 of 48) of high-risk HPV-positive samples. The entire Templex procedure, including DNA extraction, can be completed within 5 hours, providing a rapid and reliable diagnostic tool for HPV detection and typing that is amenable to automation.

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