Primary Mouse Myoblast Purification Using Magnetic Cell Separation

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2017 Mar 2

PMID 28247344

Citations 15

Authors

Marie Claude Sincennes

Yu Xin Wang

Michael A Rudnicki

Affiliations

Soon will be listed here.

Abstract

Primary myoblasts can be isolated from mouse muscle cell extracts and cultured in vitro. Muscle cells are usually dissociated manually by mincing with razor blades or scissors in a collagenase/dispase solution. Primary myoblasts are then gradually enriched by pre-plating on collagen-coated plates, based on the observation that mouse fibroblasts attach quickly to collagen-coated plates, and are less adherent. Here, we describe an automated muscle dissociation protocol. We also propose an alternative to pre-plating using magnetic bead separation of primary myoblasts, which improve myoblast purity by minimizing fibroblast contamination.

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