Analysis of Gene Expression Profiles of Induced by Direct Contact with Through Recognition of Yeast Mannan

Overview

Journal Biosci Microbiota Food Health

Specialty Biology

Date 2017 Mar 1

PMID 28243547

Citations 6

Authors

Shino Yamasaki-Yashiki

Hiroshi Sawada

Masahiro Kino-Oka

Yoshio Katakura

Affiliations

Soon will be listed here.

Abstract

Co-culture of lactic acid bacteria (LAB) and yeast induces specific responses that are not observed in pure culture. Gene expression profiles of ATCC 334 co-cultured with IFO 0216 were analyzed by DNA microarray, and the responses induced by direct contact with the yeast cells were investigated. Coating the LAB cells with recombinant DnaK, which acts as an adhesive protein between LAB and yeast cells, enhanced the ratio of adhesion of the LAB cells to the yeast cells. The signals induced by direct contact were clarified by removal of the LAB cells unbound to the yeast cells. The genes induced by direct contact with heat-inactivated yeast cells were very similar to both those induced by the intact yeast cells and those induced by a soluble mannan. The top 20 genes upregulated by direct contact with the heat-inactivated yeast cells mainly encoded proteins related to exopolysaccharide synthesis, modification of surface proteins, and transport systems. In the case of the most upregulated gene, LSEI_0669, encoding a protein that has a region homologous to polyprenyl glycosylphosphotransferase, the expression level was upregulated 7.6-, 11.0-, and 8.8-fold by the heat-inactivated yeast cells, the intact yeast cells, and the soluble mannan, respectively, whereas it was only upregulated 1.8-fold when the non-adherent LAB cells were not removed before RNA extraction. Our results indicated that the LAB responded to direct contact with the yeast cells through recognition of mannan on the surface of the yeast.

Citing Articles

Methods for Detection, Extraction, Purification, and Characterization of Exopolysaccharides of Lactic Acid Bacteria-A Systematic Review.

Yadav M, Song J, Vasquez R, Lee J, Kim I, Kang D Foods. 2024; 13(22).

PMID: 39594102 PMC: 11594216. DOI: 10.3390/foods13223687.

Acetate modulates the inhibitory effect of against the pathogenic yeasts and .

Pedro N, Fontebasso G, Pinto S, Alves M, Mira N Microb Cell. 2023; 10(4):88-102.

PMID: 37009625 PMC: 10054710. DOI: 10.15698/mic2023.04.795.

Tradition as a Stepping Stone for a Microbial Defined Water Kefir Fermentation Process: Insights in Cell Growth, Bioflavoring, and Sensory Perception.

Kohler S, Schmacht M, Troubounis A, Ludszuweit M, Rettberg N, Senz M Front Microbiol. 2022; 12:732019.

PMID: 35910583 PMC: 9336596. DOI: 10.3389/fmicb.2021.732019.

Exopolysaccharide production by lactic acid bacteria: the manipulation of environmental stresses for industrial applications.

Nguyen P, Nguyen T, Bui D, Hong P, Hoang Q, Nguyen H AIMS Microbiol. 2020; 6(4):451-469.

PMID: 33364538 PMC: 7755584. DOI: 10.3934/microbiol.2020027.

Fermentation of Wheat Bran and Whey Permeate by Mono-Cultures of Strains and Co-culture With Yeast Enhances Bioactive Properties.

Bertsch A, Roy D, LaPointe G Front Bioeng Biotechnol. 2020; 8:956.

PMID: 32850769 PMC: 7427622. DOI: 10.3389/fbioe.2020.00956.

References

Hirokawa T, Mitaku S . SOSUI: classification and secondary structure prediction system for membrane proteins. Bioinformatics. 1998; 14(4):378-9. DOI: 10.1093/bioinformatics/14.4.378. View

Mugula J, Narvhus J, Sorhaug T . Use of starter cultures of lactic acid bacteria and yeasts in the preparation of togwa, a Tanzanian fermented food. Int J Food Microbiol. 2003; 83(3):307-18. DOI: 10.1016/s0168-1605(02)00386-0. View

Marraffini L, DeDent A, Schneewind O . Sortases and the art of anchoring proteins to the envelopes of gram-positive bacteria. Microbiol Mol Biol Rev. 2006; 70(1):192-221. PMC: 1393253. DOI: 10.1128/MMBR.70.1.192-221.2006. View

Tada S, Katakura Y, Ninomiya K, Shioya S . Fed-batch coculture of Lactobacillus kefiranofaciens with Saccharomyces cerevisiae for effective production of kefiran. J Biosci Bioeng. 2007; 103(6):557-62. DOI: 10.1263/jbb.103.557. View

Hardy H, Harris J, Lyon E, Beal J, Foey A . Probiotics, prebiotics and immunomodulation of gut mucosal defences: homeostasis and immunopathology. Nutrients. 2013; 5(6):1869-912. PMC: 3725482. DOI: 10.3390/nu5061869. View

Macpherson A, Harris N . Interactions between commensal intestinal bacteria and the immune system. Nat Rev Immunol. 2004; 4(6):478-85. DOI: 10.1038/nri1373. View

Dramsi S, Trieu-Cuot P, Bierne H . Sorting sortases: a nomenclature proposal for the various sortases of Gram-positive bacteria. Res Microbiol. 2005; 156(3):289-97. DOI: 10.1016/j.resmic.2004.10.011. View

Furukawa S, Nojima N, Yoshida K, Hirayama S, Ogihara H, Morinaga Y . The importance of inter-species cell-cell co-aggregation between Lactobacillus plantarum ML11-11 and Saccharomyces cerevisiae BY4741 in mixed-species biofilm formation. Biosci Biotechnol Biochem. 2011; 75(8):1430-4. DOI: 10.1271/bbb.100817. View

Narvhus J, Gadaga T . The role of interaction between yeasts and lactic acid bacteria in African fermented milks: a review. Int J Food Microbiol. 2003; 86(1-2):51-60. DOI: 10.1016/s0168-1605(03)00247-2. View

10.

von Ossowski I, Satokari R, Reunanen J, Lebeer S, de Keersmaecker S, Vanderleyden J . Functional characterization of a mucus-specific LPXTG surface adhesin from probiotic Lactobacillus rhamnosus GG. Appl Environ Microbiol. 2011; 77(13):4465-72. PMC: 3127685. DOI: 10.1128/AEM.02497-10. View

11.

Cheirsilp B, Shoji H, Shimizu H, Shioya S . Interactions between Lactobacillus kefiranofaciens and Saccharomyces cerevisiae in mixed culture for kefiran production. J Biosci Bioeng. 2005; 96(3):279-84. DOI: 10.1016/s1389-1723(03)80194-9. View

12.

Katakura Y, Sano R, Hashimoto T, Ninomiya K, Shioya S . Lactic acid bacteria display on the cell surface cytosolic proteins that recognize yeast mannan. Appl Microbiol Biotechnol. 2009; 86(1):319-26. DOI: 10.1007/s00253-009-2295-y. View

13.

van Pijkeren J, Canchaya C, Ryan K, Li Y, Claesson M, Sheil B . Comparative and functional analysis of sortase-dependent proteins in the predicted secretome of Lactobacillus salivarius UCC118. Appl Environ Microbiol. 2006; 72(6):4143-53. PMC: 1489637. DOI: 10.1128/AEM.03023-05. View

14.

Gobbetti M, Corsetti A, Rossi J . The sourdough microflora. Interactions between lactic acid bacteria and yeasts: metabolism of amino acids. World J Microbiol Biotechnol. 2014; 10(3):275-9. DOI: 10.1007/BF00414862. View

15.

Roe J . The determination of dextran in blood and urine with anthrone reagent. J Biol Chem. 1954; 208(2):889-96. View

16.

Gadaga T, Mutukumira A, Narvhus J . The growth and interaction of yeasts and lactic acid bacteria isolated from Zimbabwean naturally fermented milk in UHT milk. Int J Food Microbiol. 2001; 68(1-2):21-32. DOI: 10.1016/s0168-1605(01)00466-4. View

17.

Viljoen B . The interaction between yeasts and bacteria in dairy environments. Int J Food Microbiol. 2001; 69(1-2):37-44. DOI: 10.1016/s0168-1605(01)00570-0. View

18.

Jensen H, Roos S, Jonsson H, Rud I, Grimmer S, van Pijkeren J . Role of Lactobacillus reuteri cell and mucus-binding protein A (CmbA) in adhesion to intestinal epithelial cells and mucus in vitro. Microbiology (Reading). 2014; 160(Pt 4):671-681. PMC: 7336543. DOI: 10.1099/mic.0.073551-0. View

19.

Tiukova I, Eberhard T, Passoth V . Interaction of Lactobacillus vini with the ethanol-producing yeasts Dekkera bruxellensis and Saccharomyces cerevisiae. Biotechnol Appl Biochem. 2013; 61(1):40-4. PMC: 4033568. DOI: 10.1002/bab.1135. View

20.

Sekirov I, Russell S, Antunes L, Finlay B . Gut microbiota in health and disease. Physiol Rev. 2010; 90(3):859-904. DOI: 10.1152/physrev.00045.2009. View