In Vitro Differentiation of Fertile Sperm from Cryopreserved Spermatogonia of the Endangered Endemic Cyprinid Honmoroko (Gnathopogon Caerulescens)

Overview

Journal Sci Rep

Specialty Science

Date 2017 Feb 18

PMID 28211534

Citations 5

Authors

Shogo Higaki

Manami Shimada

Kazuaki Kawamoto

Takaaki Todo

Toshihiro Kawasaki

Ikuo Tooyama

Yasuhiro Fujioka

Noriyoshi Sakai

Tatsuyuki Takada

Affiliations

Soon will be listed here.

Abstract

Many endemic fish species are threatened with extinction. Conservation strategies and the restoration of endemic fish after extinction must therefore be investigated. Although sperm cryopreservation is indispensable for the conservation of endangered fishes, the limited number of mature fish and limited availability (volume and period) of sperm from small endemic fish hinders the optimization and practical use of this material. In this report, we demonstrate the in vitro differentiation of fertile sperm from cryopreserved spermatogonia of juveniles of the endangered small cyprinid honmoroko (Gnathopogon caerulescens), which is endemic to Lake Biwa in Japan. The entire process of spermatogenesis was recapitulated in vitro using cryopreserved spermatogonia of non-spawning adult and juvenile fish. The differentiation of sperm from spermatogonia was captured as a time-lapse video and confirmed by 5-ethynyl-2'-deoxyuridine (EdU) incorporation into sperm. Fertility was demonstrated by artificial insemination. These results suggest that the combination of cryopreservation of spermatogonia and in vitro sperm differentiation will provide a new and promising strategy for the preservation of paternal genetic materials.

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