The Translation of Lipid Profiles to Nutritional Biomarkers in the Study of Infant Metabolism

Overview

Journal Metabolomics

Publisher Springer

Specialty Endocrinology

Date 2017 Feb 14

PMID 28190990

Citations 21

Authors

Animesh Acharjee

Philippa Prentice

Carlo Acerini

James Smith

Ieuan A Hughes

Ken Ong

Julian L Griffin

David Dunger

Albert Koulman

Affiliations

Soon will be listed here.

Abstract

Introduction: Links between early life exposures and later health outcomes may, in part, be due to in infancy. This hypothesis is supported by observed long-term benefits associated with breastfeeding, such as better cognitive development in childhood, and lower risks of obesity and high blood pressure in later life. However, the possible underlying mechanisms are expected to be complex and may be difficult to disentangle due to the lack of understanding of the metabolic processes that differentiate breastfed infants compared to those receiving just formula feed.

Objective: Our aim was to investigate the relationships between infant feeding and the lipid profiles and to validate specific lipids in separate datasets so that a small set of lipids can be used as nutritional biomarkers.

Method: We utilized a direct infusion high-resolution mass spectrometry method to analyse the lipid profiles of 3.2 mm dried blood spot samples collected at age 3 months from the Cambridge Baby Growth Study (CBGS-1), which formed the discovery cohort. For validation two sample sets were profiled: Cambridge Baby Growth Study (CBGS-2) and Pregnancy Outcome Prediction Study (POPS). Lipidomic profiles were compared between infant groups who were either exclusively breastfed, exclusively formula-fed or mixed-fed at various levels. Data analysis included supervised Random Forest method with combined classification and regression mode. Selection of lipids was based on an iterative backward elimination procedure without compromising the class error in the classification mode.

Conclusion: From this study, we were able to identify and validate three lipids: PC(35:2), SM(36:2) and SM(39:1) that can be used collectively as biomarkers for infant nutrition during early development. These biomarkers can be used to determine whether young infants (3-6 months) are breast-fed or receive formula milk.

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