Development of a Platform for Breeding by Design of CMS Restorer Lines Based on an SSSL Library in Rice ( L.)

Overview

Journal Breed Sci

Date 2017 Feb 7

PMID 28163593

Citations 17

Authors

Ziju Dai

Qing Lu

Xin Luan

Lian Ouyang

Jie Guo

Jiayan Liang

Haitao Zhu

Wenjuan Wang

Shaokui Wang

Ruizhen Zeng

Ziqiang Liu

Zemin Zhang

Xiaoyuan Zhu

Guiquan Zhang

Affiliations

Soon will be listed here.

Abstract

Exploitation of the heterosis of hybrid rice has shown great success in the improvement of rice yields. However, few genotypes exhibit strong restoration ability as effective restorers of cytoplasmic male sterility (CMS) in the development of hybrid rice. In this study, we developed a platform for the breeding by design of CMS restorer lines based on a library of chromosomal single segment substitution lines (SSSLs) in the Huajingxian74 (HJX74) genetic background. The target genes for breeding by design, and , which are associated with a strong restoration ability, and , , and , which are associated with good grain quality, were selected from the HJX74 SSSL library. Through pyramiding of the target genes, a restorer line, H121R, was developed. The H121R line was then improved regarding blast resistance by pyramiding of the gene. Hence, a new restorer line with blast resistance, H131R, was developed. The platform involving the and restorer genes would be used for the continuous improvement of restorer lines through breeding by design in rice.

Citing Articles

Improvement of the outcrossing ability of CMS lines by pyramiding QTLs for SER.

Tan Q, Gan Z, Shao L, Xiong L, Luan X, Yang W Mol Breed. 2025; 45(2):24.

PMID: 39931133 PMC: 11807039. DOI: 10.1007/s11032-025-01545-5.

Four QTLs control stigma exsertion rate by changing stigma size in rice.

Tan Q, Gan Z, Xiong L, Shao L, Yang W, Luan X Mol Breed. 2024; 44(9):59.

PMID: 39263271 PMC: 11383900. DOI: 10.1007/s11032-024-01499-0.

Genetic Effects of Grain Quality Enhancement in Indica Hybrid Rice: Insights for Molecular Design Breeding.

Gao J, Gao L, Chen W, Huang J, Qing D, Pan Y Rice (N Y). 2024; 17(1):39.

PMID: 38874692 PMC: 11178727. DOI: 10.1186/s12284-024-00719-7.

Pyramiding of Low Chalkiness QTLs Is an Effective Way to Reduce Rice Chalkiness.

Yang W, Chen S, Hao Q, Zhu H, Tan Q, Lin S Rice (N Y). 2024; 17(1):4.

PMID: 38185771 PMC: 10772014. DOI: 10.1186/s12284-023-00680-x.

Establishment and Advances of Third-Generation Hybrid Rice Technology: A Review.

Chen Z, Wu J, Deng X, Tang X Rice (N Y). 2023; 16(1):56.

PMID: 38063909 PMC: 10709537. DOI: 10.1186/s12284-023-00670-z.

References

Wang Z, Zou Y, Li X, Zhang Q, Chen L, Wu H . Cytoplasmic male sterility of rice with boro II cytoplasm is caused by a cytotoxic peptide and is restored by two related PPR motif genes via distinct modes of mRNA silencing. Plant Cell. 2006; 18(3):676-87. PMC: 1383642. DOI: 10.1105/tpc.105.038240. View

Cheng S, Zhuang J, Fan Y, Du J, Cao L . Progress in research and development on hybrid rice: a super-domesticate in China. Ann Bot. 2007; 100(5):959-66. PMC: 2759200. DOI: 10.1093/aob/mcm121. View

Hu J, Wang K, Huang W, Liu G, Gao Y, Wang J . The rice pentatricopeptide repeat protein RF5 restores fertility in Hong-Lian cytoplasmic male-sterile lines via a complex with the glycine-rich protein GRP162. Plant Cell. 2012; 24(1):109-22. PMC: 3289560. DOI: 10.1105/tpc.111.093211. View

Tan Y, Li J, Yu S, Xing Y, Xu C, Zhang Q . The three important traits for cooking and eating quality of rice grains are controlled by a single locus in an elite rice hybrid, Shanyou 63. Theor Appl Genet. 2012; 99(3-4):642-8. DOI: 10.1007/s001220051279. View

Liu G, Zhu H, Zhang G, Li L, Ye G . Dynamic analysis of QTLs on tiller number in rice (Oryza sativa L.) with single segment substitution lines. Theor Appl Genet. 2012; 125(1):143-53. DOI: 10.1007/s00122-012-1822-x. View

Xie J, Zhuang J, Fan Y, Tu G, Xia Y, Zheng K . [Mapping of fertility-restoring genes with main effects and epistatic effects for CMS-DA in rice]. Yi Chuan Xue Bao. 2002; 29(7):616-21. View

Chen L, Liu Y . Male sterility and fertility restoration in crops. Annu Rev Plant Biol. 2013; 65:579-606. DOI: 10.1146/annurev-arplant-050213-040119. View

Liu G, Zhu H, Liu S, Zeng R, Zhang Z, Li W . Unconditional and conditional QTL mapping for the developmental behavior of tiller number in rice (Oryza sativa L.). Genetica. 2010; 138(8):885-93. DOI: 10.1007/s10709-010-9471-y. View

Fan C, Xing Y, Mao H, Lu T, Han B, Xu C . GS3, a major QTL for grain length and weight and minor QTL for grain width and thickness in rice, encodes a putative transmembrane protein. Theor Appl Genet. 2006; 112(6):1164-71. DOI: 10.1007/s00122-006-0218-1. View

10.

Murray M, Thompson W . Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 1980; 8(19):4321-5. PMC: 324241. DOI: 10.1093/nar/8.19.4321. View

11.

Zhang G, Lu Y, Bharaj T, Virmani S, Huang N . Mapping of the Rf-3 nuclear fertility-restoring gene for WA cytoplasmic male sterility in rice using RAPD and RFLP markers. Theor Appl Genet. 2009; 94(1):27-33. DOI: 10.1007/s001220050377. View

12.

Kazama T, Toriyama K . A pentatricopeptide repeat-containing gene that promotes the processing of aberrant atp6 RNA of cytoplasmic male-sterile rice. FEBS Lett. 2003; 544(1-3):99-102. DOI: 10.1016/s0014-5793(03)00480-0. View

13.

Gao Z, Zeng D, Cui X, Zhou Y, Yan M, Huang D . Map-based cloning of the ALK gene, which controls the gelatinization temperature of rice. Sci China C Life Sci. 2008; 46(6):661-8. DOI: 10.1360/03yc0099. View

14.

Zhang Q, Liu Y, Zhang G, Mei M . [Molecular mapping of the fertility restorer gene Rf-4 for WA cytoplasmic male sterility in rice]. Yi Chuan Xue Bao. 2003; 29(11):1001-4. View

15.

Luo D, Xu H, Liu Z, Guo J, Li H, Chen L . A detrimental mitochondrial-nuclear interaction causes cytoplasmic male sterility in rice. Nat Genet. 2013; 45(5):573-7. DOI: 10.1038/ng.2570. View

16.

Peleman J, Rouppe van der Voort J . Breeding by design. Trends Plant Sci. 2003; 8(7):330-4. DOI: 10.1016/S1360-1385(03)00134-1. View

17.

Zhu H, Liu Z, Fu X, Dai Z, Wang S, Zhang G . Detection and characterization of epistasis between QTLs on plant height in rice using single segment substitution lines. Breed Sci. 2015; 65(3):192-200. PMC: 4482168. DOI: 10.1270/jsbbs.65.192. View

18.

Chen J, Li X, Cheng C, Wang Y, Qin M, Zhu H . Characterization of epistatic interaction of QTLs LH8 and EH3 controlling heading date in rice. Sci Rep. 2014; 4:4263. PMC: 3939452. DOI: 10.1038/srep04263. View

19.

Liu G, Zhang Z, Zhu H, Zhao F, Ding X, Zeng R . Detection of QTLs with additive effects and additive-by-environment interaction effects on panicle number in rice (Oryza sativa L.) with single-segment substitution lines. Theor Appl Genet. 2008; 116(7):923-31. DOI: 10.1007/s00122-008-0724-4. View

20.

Wang S, Wu K, Yuan Q, Liu X, Liu Z, Lin X . Control of grain size, shape and quality by OsSPL16 in rice. Nat Genet. 2012; 44(8):950-4. DOI: 10.1038/ng.2327. View