IL-7 Induces an Epitope Masking of C Protein in IL-7 Receptor Signaling Complex

Overview

Journal Mediators Inflamm

Publisher Wiley

Specialties Biochemistry
Pathology

Date 2017 Jan 28

PMID 28127156

Citations 2

Authors

Tae Sik Goh

Yuna Jo

Byunghyuk Lee

Geona Kim

Hyunju Hwang

Eunhee Ko

Seung Wan Kang

Sae-Ock Oh

Sun-Yong Baek

Sik Yoon

Jung Sub Lee

Changwan Hong

Affiliations

Soon will be listed here.

Abstract

IL-7 signaling via IL-7R and common -chain (c) is necessary for the development and homeostasis of T cells. Although the delicate mechanism in which IL-7R downregulation allows the homeostasis of T cell with limited IL-7 has been well known, the exact mechanism behind the interaction between IL-7R and c in the absence or presence of IL-7 remains unclear. Additionally, we are still uncertain as to how only IL-7R is separately downregulated by the binding of IL-7 from the IL-7R/c complex. We demonstrate here that 4G3, TUGm2, and 3E12 epitope masking of c protein are induced in the presence of IL-7, indicating that the epitope alteration is induced by IL-7 binding to the preassembled receptor core. Moreover, the epitope masking of c protein is inversely correlated with the expression of IL-7R upon IL-7 binding, implying that the structural alteration of c might be involved in the regulation of IL-7R expression. The conformational change in c upon IL-7 binding may contribute not only to forming the functional IL-7 signaling complex but also to optimally regulating the expression of IL-7R.

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