Simultaneous Determination of Lactate, Pyruvate, and Ascorbate in Microdialysis Samples from Rat Brain, Blood, Fat, and Muscle Using High-performance Liquid Chromatography

Overview

Journal J Pharmacol Methods

Specialties Pharmacology
Toxicology

Date 1989 Sep 1

PMID 2811388

Citations 15

Authors

A Hallstrom

A Carlsson

L Hillered

U Ungerstedt

Affiliations

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Abstract

An isocratic high-performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of lactate, pyruvate, and ascorbate in tissue and blood, sampled in vivo using the microdialysis technique. The compounds were separated on a silica-based, reversed-phase column or on a resin-based, cation exchange column. The acids were monitored by ultraviolet detection.

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