Overview of Brain Microdialysis

Overview

Journal Curr Protoc Neurosci

Date 2009 Apr 3

PMID 19340812

Citations 134

Authors

Vladimir I Chefer

Alexis C Thompson

Agustin Zapata

Toni S Shippenberg

Affiliations

Soon will be listed here.

Abstract

The technique of microdialysis enables sampling and collecting of small-molecular-weight substances from the interstitial space. It is a widely used method in neuroscience and is one of the few techniques available that permits quantification of neurotransmitters, peptides, and hormones in the behaving animal. More recently, it has been used in tissue preparations for quantification of neurotransmitter release. This unit provides a brief review of the history of microdialysis and its general application in the neurosciences. The authors review the theoretical principles underlying the microdialysis process, methods available for estimating extracellular concentration from dialysis samples (i.e., relative recovery), the various factors that affect the estimate of in vivo relative recovery, and the importance of determining in vivo relative recovery to data interpretation. Several areas of special note, including impact of tissue trauma on the interpretation of microdialysis results, are discussed. Step-by-step instructions for the planning and execution of conventional and quantitative microdialysis experiments are provided.

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References

Sato Y, Shibanoki S, Sugahara M, Ishikawa K . Measurement and pharmacokinetic analysis of imipramine and its metabolite by brain microdialysis. Br J Pharmacol. 1994; 112(2):625-9. PMC: 1910334. DOI: 10.1111/j.1476-5381.1994.tb13120.x. View

Cebada J, Alvarado-Alvarez R, Becerra E, Neri-Bazan L, Rocha L, Garcia U . An improved method for long-term measuring of hemolymph fluctuations of non-essential amino acids, GABA and histamine from freely moving crayfish. J Neurosci Methods. 2006; 153(1):1-7. DOI: 10.1016/j.jneumeth.2005.05.025. View

Chen K . Effects of tissue trauma on the characteristics of microdialysis zero-net-flux method sampling neurotransmitters. J Theor Biol. 2005; 238(4):863-81. DOI: 10.1016/j.jtbi.2005.06.035. View

Huang Y, Zielke C, Tildon J, Zielke H . Monitoring in vivo oxidation of 14C-labelled substrates to 14CO2 by brain microdialysis. Dev Neurosci. 1993; 15(3-5):233-9. DOI: 10.1159/000111339. View

Keefe K, Sved A, Zigmond M, Abercrombie E . Stress-induced dopamine release in the neostriatum: evaluation of the role of action potentials in nigrostriatal dopamine neurons or local initiation by endogenous excitatory amino acids. J Neurochem. 1993; 61(5):1943-52. DOI: 10.1111/j.1471-4159.1993.tb09837.x. View

Nomoto M, Irifune M, Fukuzaki K, Fukuda T . Effects of bifemelane on parkinsonism induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in the common marmoset. Neurosci Lett. 1994; 178(1):95-8. DOI: 10.1016/0304-3940(94)90298-4. View

Nordenvall M, Ulmsten U, Ungerstedt U . Influence of progesterone on the sodium and potassium concentrations of rat uterine fluid investigated by microdialysis. Gynecol Obstet Invest. 1989; 28(2):73-7. DOI: 10.1159/000293518. View

Westerink B, Hofsteede H, Damsma G, DE VRIES J . The significance of extracellular calcium for the release of dopamine, acetylcholine and amino acids in conscious rats, evaluated by brain microdialysis. Naunyn Schmiedebergs Arch Pharmacol. 1988; 337(4):373-8. DOI: 10.1007/BF00169526. View

Lindefors N, Amberg G, Ungerstedt U . Intracerebral microdialysis: I. Experimental studies of diffusion kinetics. J Pharmacol Methods. 1989; 22(3):141-56. DOI: 10.1016/0160-5402(89)90011-9. View

10.

Westerink B . Analysis of biogenic amines in microdialysates of the brain. J Chromatogr B Biomed Sci Appl. 2000; 747(1-2):21-32. DOI: 10.1016/s0378-4347(00)00338-8. View

11.

Mason P, Romano W . Recovery characteristics of a rigid, nonmetallic microdialysis probe for use in an electromagnetic field. Bioelectromagnetics. 1995; 16(2):113-8. DOI: 10.1002/bem.2250160207. View

12.

Dev B, Mason P, Freed C . Drug-induced changes in blood pressure lead to changes in extracellular concentrations of epinephrine, dihydroxyphenylacetic acid, and 5-hydroxyindoleacetic acid in the rostral ventrolateral medulla of the rat. J Neurochem. 1992; 58(4):1386-94. DOI: 10.1111/j.1471-4159.1992.tb11354.x. View

13.

Venero C, Borrell J . Rapid glucocorticoid effects on excitatory amino acid levels in the hippocampus: a microdialysis study in freely moving rats. Eur J Neurosci. 1999; 11(7):2465-73. DOI: 10.1046/j.1460-9568.1999.00668.x. View

14.

During M, Bean A, Roth R . Effects of CNS stimulants on the in vivo release of the colocalized transmitters, dopamine and neurotensin, from rat prefrontal cortex. Neurosci Lett. 1992; 140(1):129-33. DOI: 10.1016/0304-3940(92)90698-7. View

15.

Ferraro T, Weyers P, Carrozza D, Vogel W . Continuous monitoring of brain ethanol levels by intracerebral microdialysis. Alcohol. 1990; 7(2):129-32. DOI: 10.1016/0741-8329(90)90073-l. View

16.

Yue T, Gu J, Lysko P, Cheng H, Barone F, Feuerstein G . Neuroprotective effects of phenyl-t-butyl-nitrone in gerbil global brain ischemia and in cultured rat cerebellar neurons. Brain Res. 1992; 574(1-2):193-7. DOI: 10.1016/0006-8993(92)90816-r. View

17.

Delgado J, Defeudis F, Roth R, Ryugo D, Mitruka B . Dialytrode for long term intracerebral perfusion in awake monkeys. Arch Int Pharmacodyn Ther. 1972; 198(1):9-21. View

18.

Westerink B, Damsma G, Rollema H, DE VRIES J, Horn A . Scope and limitations of in vivo brain dialysis: a comparison of its application to various neurotransmitter systems. Life Sci. 1987; 41(15):1763-76. DOI: 10.1016/0024-3205(87)90695-3. View

19.

Lonnroth P, Strindberg L . Validation of the 'internal reference technique' for calibrating microdialysis catheters in situ. Acta Physiol Scand. 1995; 153(4):375-80. DOI: 10.1111/j.1748-1716.1995.tb09875.x. View

20.

Stahle L, Segersvard S, Ungerstedt U . A comparison between three methods for estimation of extracellular concentrations of exogenous and endogenous compounds by microdialysis. J Pharmacol Methods. 1991; 25(1):41-52. DOI: 10.1016/0160-5402(91)90021-v. View