Human Dental Pulp Stem Cells Are More Effective Than Human Bone Marrow-Derived Mesenchymal Stem Cells in Cerebral Ischemic Injury

Overview

Journal Cell Transplant

Specialties Cell Biology
General Surgery

Date 2017 Jan 21

PMID 28105979

Citations 65

Authors

Miyeoun Song

Jae-Hyung Lee

Jinhyun Bae

Youngmin Bu

Eun-Cheol Kim

Affiliations

Soon will be listed here.

Abstract

We compared the therapeutic effects and mechanism of transplanted human dental pulp stem cells (hDPSCs) and human bone marrow-derived mesenchymal stem cells (hBM-MSCs) in a rat stroke model and an in vitro model of ischemia. Rats were intravenously injected with hDPSCs or hBM-MSCs 24 h after middle cerebral artery occlusion (MCAo), and both groups showed improved functional recovery and reduced infarct volume versus control rats, but the hDPSC group showed greater reduction in infarct volume than the hBM-MSC group. The positive area for the endothelial cell marker was greater in the lesion boundary areas in the hDPSC group than in the hBM-MSC group. Administration of hDPSCs to rats with stroke significantly decreased reactive gliosis, as evidenced by the attenuation of MCAo-induced GFAP+/nestin+ and GFAP+/Musashi-1+ cells, compared with hBM-MSCs. In vivo findings were confirmed by in vitro data illustrating that hDPSCs showed superior neuroprotective, migratory, and in vitro angiogenic effects in oxygen-glucose deprivation (OGD)-injured human astrocytes (hAs) versus hBM-MSCs. Comprehensive comparative bioinformatics analyses from hDPSC- and hBM-MSC-treated in vitro OGD-injured hAs were examined by RNA sequencing technology. In gene ontology and KEGG pathway analyses, significant pathways in the hDPSC-treated group were the MAPK and TGF-β signaling pathways. Thus, hDPSCs may be a better cell therapy source for ischemic stroke than hBM-MSCs.

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References

He Y, Zhang H, Yung A, Villeda S, Jaeger P, Olayiwola O . ALK5-dependent TGF-β signaling is a major determinant of late-stage adult neurogenesis. Nat Neurosci. 2014; 17(7):943-52. PMC: 4096284. DOI: 10.1038/nn.3732. View

Onda T, Honmou O, Harada K, Houkin K, Hamada H, Kocsis J . Therapeutic benefits by human mesenchymal stem cells (hMSCs) and Ang-1 gene-modified hMSCs after cerebral ischemia. J Cereb Blood Flow Metab. 2007; 28(2):329-40. PMC: 2605394. DOI: 10.1038/sj.jcbfm.9600527. View

Pierdomenico L, Bonsi L, Calvitti M, Rondelli D, Arpinati M, Chirumbolo G . Multipotent mesenchymal stem cells with immunosuppressive activity can be easily isolated from dental pulp. Transplantation. 2005; 80(6):836-42. DOI: 10.1097/01.tp.0000173794.72151.88. View

Sugiyama M, Iohara K, Wakita H, Hattori H, Ueda M, Matsushita K . Dental pulp-derived CD31⁻/CD146⁻ side population stem/progenitor cells enhance recovery of focal cerebral ischemia in rats. Tissue Eng Part A. 2011; 17(9-10):1303-11. DOI: 10.1089/ten.TEA.2010.0306. View

Huang A, Snyder B, Cheng P, Chan A . Putative dental pulp-derived stem/stromal cells promote proliferation and differentiation of endogenous neural cells in the hippocampus of mice. Stem Cells. 2008; 26(10):2654-63. PMC: 4416417. DOI: 10.1634/stemcells.2008-0285. View

Kim H, Cho Y, Lee Y, Lee S, Bae W, Kim E . PIN1 Suppresses the Hepatic Differentiation of Pulp Stem Cells via Wnt3a. J Dent Res. 2016; 95(12):1415-1424. DOI: 10.1177/0022034516659642. View

Bakshi A, Hunter C, Swanger S, Lepore A, Fischer I . Minimally invasive delivery of stem cells for spinal cord injury: advantages of the lumbar puncture technique. J Neurosurg Spine. 2004; 1(3):330-7. DOI: 10.3171/spi.2004.1.3.0330. View

Diniz L, Matias I, Garcia M, Gomes F . Astrocytic control of neural circuit formation: highlights on TGF-beta signaling. Neurochem Int. 2014; 78:18-27. DOI: 10.1016/j.neuint.2014.07.008. View

Nomura T, Honmou O, Harada K, Houkin K, Hamada H, Kocsis J . I.V. infusion of brain-derived neurotrophic factor gene-modified human mesenchymal stem cells protects against injury in a cerebral ischemia model in adult rat. Neuroscience. 2005; 136(1):161-9. PMC: 2605391. DOI: 10.1016/j.neuroscience.2005.06.062. View

10.

Nakashima M, Iohara K, Sugiyama M . Human dental pulp stem cells with highly angiogenic and neurogenic potential for possible use in pulp regeneration. Cytokine Growth Factor Rev. 2009; 20(5-6):435-40. DOI: 10.1016/j.cytogfr.2009.10.012. View

11.

Yalvac M, Rizvanov A, Kilic E, Sahin F, Mukhamedyarov M, Islamov R . Potential role of dental stem cells in the cellular therapy of cerebral ischemia. Curr Pharm Des. 2009; 15(33):3908-16. DOI: 10.2174/138161209789649439. View

12.

Owens D, Keyse S . Differential regulation of MAP kinase signalling by dual-specificity protein phosphatases. Oncogene. 2007; 26(22):3203-13. DOI: 10.1038/sj.onc.1210412. View

13.

Zhang Z, Zhang L, Jiang Q, Chopp M . Bone marrow-derived endothelial progenitor cells participate in cerebral neovascularization after focal cerebral ischemia in the adult mouse. Circ Res. 2002; 90(3):284-8. DOI: 10.1161/hh0302.104460. View

14.

Hanel M, Hensey C . Eye and neural defects associated with loss of GDF6. BMC Dev Biol. 2006; 6:43. PMC: 1609107. DOI: 10.1186/1471-213X-6-43. View

15.

Matsushita K, Motani R, Sakuta T, Yamaguchi N, Koga T, Matsuo K . The role of vascular endothelial growth factor in human dental pulp cells: induction of chemotaxis, proliferation, and differentiation and activation of the AP-1-dependent signaling pathway. J Dent Res. 2000; 79(8):1596-603. DOI: 10.1177/00220345000790081201. View

16.

Oki K, Kaneko N, Kanki H, Imai T, Suzuki N, Sawamoto K . Musashi1 as a marker of reactive astrocytes after transient focal brain ischemia. Neurosci Res. 2009; 66(4):390-5. DOI: 10.1016/j.neures.2009.12.013. View

17.

Hilkens P, Fanton Y, Martens W, Gervois P, Struys T, Politis C . Pro-angiogenic impact of dental stem cells in vitro and in vivo. Stem Cell Res. 2014; 12(3):778-90. DOI: 10.1016/j.scr.2014.03.008. View

18.

Gronthos S, Mankani M, Brahim J, Robey P, Shi S . Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci U S A. 2000; 97(25):13625-30. PMC: 17626. DOI: 10.1073/pnas.240309797. View

19.

Anders S, Huber W . Differential expression analysis for sequence count data. Genome Biol. 2010; 11(10):R106. PMC: 3218662. DOI: 10.1186/gb-2010-11-10-r106. View

20.

Hunt J, Petroff M, McIntire R, Ober C . HLA-G and immune tolerance in pregnancy. FASEB J. 2005; 19(7):681-93. DOI: 10.1096/fj.04-2078rev. View