Live Cell Imaging of Endogenous MRNA Using RNA-Based Fluorescence "Turn-On" Probe

Overview

Journal ACS Chem Biol

Publisher American Chemical Society

Specialties Biochemistry
Biology

Date 2017 Jan 21

PMID 28103687

Citations 13

Authors

Wei Qiang Ong

Y Rose Citron

Sayaka Sekine

Bo Huang

Affiliations

Soon will be listed here.

Abstract

Messenger RNA (mRNA) plays a critical role in cellular growth and development. However, there have been limited methods available to visualize endogenous mRNA in living cells with ease. We have designed RNA-based fluorescence "turn-on" probes that target mRNA by fusing an unstable form of Spinach with target-complementary sequences. These probes have been demonstrated to be selective, stable, and capable of targeting various mRNAs for live E. coli imaging.

Citing Articles

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Turn-on RNA Mango Beacons for -acting fluorogenic nucleic acid detection.

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RNA-based fluorescent biosensors for live cell detection of bacterial sRNA.

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