Expression of Apical Junction Complex Proteins in Colorectal Mucosa of Miniature Dachshunds with Inflammatory Colorectal Polyps

Overview

Journal J Vet Med Sci

Specialty Veterinary Medicine

Date 2017 Jan 17

PMID 28090006

Authors

Nozomu Yokoyama

Hiroshi Ohta

Yumiko Kagawa

Rommaneeya Leela-Arporn

Angkhana Dermlim

Khoirun Nisa

Tomoya Morita

Tatsuyuki Osuga

Noboru Sasaki

Keitaro Morishita

Kensuke Nakamura

Mitsuyoshi Takiguchi

Affiliations

Soon will be listed here.

Abstract

We examine the expression of tight junction and adherence junction proteins in the colorectal mucosa of miniature dachshunds (MDs) with inflammatory colorectal polyps (ICRPs). Colorectal mucosa samples were endoscopically obtained from 8 MDs with ICRPs and 8 control dogs for immunoblotting. Paraffin-embedded tissues of surgically resected inflamed lesions from another 5 MDs with ICRPs and full-thickness colorectal specimens from 5 healthy beagles were obtained for immunohistochemistry. The expression patterns of claudin-1, -2, -3, -4, -5, -7 and -8, E-cadherin and β-catenin were analyzed in the non-inflamed mucosa and inflamed mucosa of ICRPs and colorectal mucosa of control dogs by immunoblotting. The localization of these proteins in the inflamed lesions was analyzed by immunohistochemistry. The expressions of each of claudin, E-cadherin and β-catenin were not significantly different between control dogs and non-inflamed colonic mucosa from MDs with ICRPs. In contrast, only E-cadherin and β-catenin were detected in the inflamed lesions of MDs with ICRPs. By immunohistochemistry, claudin-2, -3, -4, -5 and -7, E-cadherin and β-catenin were expressed in the colorectal epithelium within the inflamed mucosa, but not in granulation tissue. Distributions of claudin-2, -3, -4, -5, and -7, E-cadherin and β-catenin in the colonic epithelium were not different between MDs with ICRPs and control dogs. These results indicated that no significant alteration was detected in several tight junction or adherence junction proteins expression in the colorectal epithelium of ICRPs.

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