Microfluidic Approaches for Isolation, Detection, and Characterization of Extracellular Vesicles: Current Status and Future Directions

Overview

Journal Biosens Bioelectron

Specialties Biochemistry
Biotechnology

Date 2017 Jan 16

PMID 28088752

Citations 85

Authors

Shima Gholizadeh

Mohamed Shehata Draz

Maryam Zarghooni

Amir Sanati-Nezhad

Saeid Ghavami

Hadi Shafiee

Mohsen Akbari

Affiliations

Soon will be listed here.

Abstract

Extracellular vesicles (EVs) are cell-derived vesicles present in body fluids that play an essential role in various cellular processes, such as intercellular communication, inflammation, cellular homeostasis, survival, transport, and regeneration. Their isolation and analysis from body fluids have a great clinical potential to provide information on a variety of disease states such as cancer, cardiovascular complications and inflammatory disorders. Despite increasing scientific and clinical interest in this field, there are still no standardized procedures available for the purification, detection, and characterization of EVs. Advances in microfluidics allow for chemical sampling with increasingly high spatial resolution and under precise manipulation down to single molecule level. In this review, our objective is to give a brief overview on the working principle and examples of the isolation and detection methods with the potential to be used for extracellular vesicles. This review will also highlight the integrated on-chip systems for isolation and characterization of EVs.

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