Isolation, Identification, and Challenges of Extracellular Vesicles: Emerging Players in Clinical Applications

Overview

Journal Apoptosis

Publisher Springer

Date 2024 Nov 10

PMID 39522104

Authors

Xiaoxiao Ma

Lanwei Peng

Xiaohui Zhu

Tianqi Chu

Changcheng Yang

Bohao Zhou

Xiangwei Sun

Tianya Gao

Mengqi Zhang

Ping Chen

Haiyan Chen

Affiliations

Soon will be listed here.

Abstract

Extracellular vesicles (EVs) serve as critical mediators of intercellular communication, encompassing exosomes, microvesicles, and apoptotic vesicles that play significant roles in diverse physiological and pathological contexts. Numerous studies have demonstrated that EVs derived from mesenchymal stem cells (MSC-EVs) play a pivotal role in facilitating tissue and organ repair, alleviating inflammation and apoptosis, enhancing the proliferation of endogenous stem cells within tissues and organs, and modulating immune function-these functions have been extensively utilized in clinical applications. The precise classification, isolation, and identification of MSC-EVs are essential for their clinical applications. This article provides a comprehensive overview of the biological properties of EVs, emphasizing both their advantages and limitations in isolation and identification methodologies. Additionally, we summarize the protein markers associated with MSC-EVs, emphasizing their significance in the treatment of various diseases. Finally, this article addresses the current challenges and dilemmas in developing clinical applications for MSC-EVs, aiming to offer valuable insights for future research.

Citing Articles

Extracellular Vesicles as Mediators of Endothelial Dysfunction in Cardiovascular Diseases.

Jimenez-Trinidad F, Calvo-Gomez S, Sabate M, Brugaletta S, Campuzano V, Egea G Int J Mol Sci. 2025; 26(3).

PMID: 39940780 PMC: 11816526. DOI: 10.3390/ijms26031008.

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