High-resolution Sub-cellular Imaging by Correlative NanoSIMS and Electron Microscopy of Amiodarone Internalisation by Lung Macrophages As Evidence for Drug-induced Phospholipidosis

Overview

Journal Chem Commun (Camb)

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2017 Jan 14

PMID 28085162

Citations 14

Authors

Haibo Jiang

Melissa K Passarelli

Peter M G Munro

Matt R Kilburn

Andrew West

Colin T Dollery

Ian S Gilmore

Paulina D Rakowska

Affiliations

Soon will be listed here.

Abstract

Correlative NanoSIMS and EM imaging of amiodarone-treated macrophages shows the internalisation of the drug at a sub-cellular level and reveals its accumulation within the lysosomes, providing direct evidence for amiodarone-induced phospholipidosis. Chemical fixation using tannic acid effectively seals cellular membranes aiding intracellular retention of diffusible drugs.

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