CircRNA_000203 Enhances the Expression of Fibrosis-associated Genes by Derepressing Targets of MiR-26b-5p, Col1a2 and CTGF, in Cardiac Fibroblasts

Overview

Journal Sci Rep

Specialty Science

Date 2017 Jan 13

PMID 28079129

Citations 135

Authors

Chun-Mei Tang

Ming Zhang

Lei Huang

Zhi-Qin Hu

Jie-Ning Zhu

Zhen Xiao

Zhuo Zhang

Qiu-Xiong Lin

Xi-Long Zheng

Min -Yang

Shu-Lin Wu

Jian-Ding Cheng

Zhi-Xin Shan

Affiliations

Soon will be listed here.

Abstract

Circular RNAs (circRNAs) participate in regulating gene expression in diverse biological and pathological processes. The present study aimed to investigate the mechanism underlying the modulation of circRNA_000203 on expressions of fibrosis-associated genes in cardiac fibroblasts. CircRNA_000203 was shown upregulated in the diabetic mouse myocardium and in Ang-II-induced mouse cardiac fibroblasts. Enforced-expression of circRNA_000203 could increase expressions of Col1a2, Col3a1 and α-SMA in mouse cardiac fibroblasts. RNA pull-down and RT-qPCR assay indicated that circRNA_000203 could specifically sponge miR-26b-5p. Dual luciferase reporter assay revealed that miR-26b-5p interacted with 3'UTRs of Col1a2 and CTGF, and circ_000203 could block the interactions of miR-26b-5p and 3'UTRs of Col1a2 and CTGF. Transfection of miR-26b-5p could post-transcriptionaly inhibit expressions of Col1a2 and CTGF, accompanied with the suppressions of Col3a1 and α-SMA in cardiac fibroblasts. Additionally, over-expression of circRNA_000203 could eliminate the anti-fibrosis effect of miR-26b-5p in cardiac fibroblasts. Together, our results reveal that suppressing the function of miR-26b-5p contributes to the pro-fibrosis effect of circRNA_000203 in cardiac fibroblasts.

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