Generating Exome Enriched Sequencing Libraries from Formalin-Fixed, Paraffin-Embedded Tissue DNA for Next-Generation Sequencing

Overview

Journal Curr Protoc Hum Genet

Specialty Genetics

Date 2017 Jan 12

PMID 28075488

Citations 3

Authors

Beth A Marosy

Brian D Craig

Kurt N Hetrick

P Dane Witmer

Hua Ling

Sean M Griffith

Benjamin Myers

Elaine A Ostrander

Janet L Stanford

Lawrence C Brody

Kimberly F Doheny

Affiliations

Soon will be listed here.

Abstract

This unit describes a technique for generating exome-enriched sequencing libraries using DNA extracted from formalin-fixed paraffin-embedded (FFPE) samples. Utilizing commercially available kits, we present a low-input FFPE workflow starting with 50 ng of DNA. This procedure includes a repair step to address damage caused by FFPE preservation that improves sequence quality. Subsequently, libraries undergo an in-solution-targeted selection for exons, followed by sequencing using the Illumina next-generation short-read sequencing platform. © 2017 by John Wiley & Sons, Inc.

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