Elasto-inertial Microfluidics for Bacteria Separation from Whole Blood for Sepsis Diagnostics

Overview

Journal J Nanobiotechnology

Publisher Biomed Central

Specialty Biotechnology

Date 2017 Jan 6

PMID 28052769

Citations 26

Authors

Muhammad Asim Faridi

Harisha Ramachandraiah

Indradumna Banerjee

Sahar Ardabili

Sergey Zelenin

Aman Russom

Affiliations

Soon will be listed here.

Abstract

Background: Bloodstream infections (BSI) remain a major challenge with high mortality rate, with an incidence that is increasing worldwide. Early treatment with appropriate therapy can reduce BSI-related morbidity and mortality. However, despite recent progress in molecular based assays, complex sample preparation steps have become critical roadblock for a greater expansion of molecular assays. Here, we report a size based, label-free, bacteria separation from whole blood using elasto-inertial microfluidics.

Results: In elasto-inertial microfluidics, the viscoelastic flow enables size based migration of blood cells into a non-Newtonian solution, while smaller bacteria remain in the streamline of the blood sample entrance and can be separated. We first optimized the flow conditions using particles, and show continuous separation of 5 μm particles from 2 μm at a yield of 95% for 5 µm particle and 93% for 2 µm particles at respective outlets. Next, bacteria were continuously separated at an efficiency of 76% from undiluted whole blood sample.

Conclusion: We demonstrate separation of bacteria from undiluted while blood using elasto-inertial microfluidics. The label-free, passive bacteria preparation method has a great potential for downstream phenotypic and molecular analysis of bacteria.

Citing Articles

Viscoelastic particle focusing and separation in a microfluidic channel with a cruciform section.

Jang J, Ahn J, Kim T, Cho Y Biomicrofluidics. 2024; 18(6):064101.

PMID: 39553922 PMC: 11567695. DOI: 10.1063/5.0233177.

Elasto-inertial focusing and particle migration in high aspect ratio microchannels for high-throughput separation.

Tanriverdi S, Cruz J, Habibi S, Amini K, Costa M, Lundell F Microsyst Nanoeng. 2024; 10:87.

PMID: 38919163 PMC: 11196675. DOI: 10.1038/s41378-024-00724-2.

Cascaded signal amplification strategy for ultra-specific, ultra-sensitive, and visual detection of Shigella flexneri.

Shi Y, Tan Q, Gong T, Li Q, Zhu Y, Duan X Mikrochim Acta. 2024; 191(5):271.

PMID: 38632191 DOI: 10.1007/s00604-024-06309-0.

Blood component separation in straight microfluidic channels.

Lee L, Bhatt K, Haithcock D, Prabhakarpandian B Biomicrofluidics. 2023; 17(5):054106.

PMID: 37854890 PMC: 10581738. DOI: 10.1063/5.0176457.

Design and Fabrication of a High Performance Microfluidic Chip for Blood Plasma Separation: Modelling and Prediction of System Behaviour via CFD Method.

Amini H, Sokhansanj A, Akrami M, Haririan I Int J Anal Chem. 2023; 2023:3648247.

PMID: 37404341 PMC: 10317604. DOI: 10.1155/2023/3648247.

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