The "PepSAVI-MS" Pipeline for Natural Product Bioactive Peptide Discovery

Overview

Journal Anal Chem

Specialty Chemistry

Date 2016 Dec 20

PMID 27991763

Citations 23

Authors

Christine L Kirkpatrick

Christopher A Broberg

Elijah N McCool

Woo Jean Lee

Alex Chao

Evan W McConnell

David A Pritchard

Michael Hebert

Renee Fleeman

Jessie Adams

Amer Jamil

Laurence Madera

Adam A Stromstedt

Ulf Goransson

Yufeng Liu

David W Hoskin

Lindsey N Shaw

Leslie M Hicks

Affiliations

Soon will be listed here.

Abstract

The recent increase in extensively drug-resistant bacterial pathogens and the associated increase of morbidity and mortality demonstrate the immediate need for new antibiotic backbones with novel mechanisms of action. Here, we report the development of the PepSAVI-MS pipeline for bioactive peptide discovery. This highly versatile platform employs mass spectrometry and statistics to identify bioactive peptide targets from complex biological samples. We validate the use of this platform through the successful identification of known bioactive peptides from a botanical species, Viola odorata. Using this pipeline, we have widened the known antimicrobial spectrum for V. odorata cyclotides, including antibacterial activity of cycloviolacin O2 against A. baumannii. We further demonstrate the broad applicability of the platform through the identification of novel anticancer activities for cycloviolacins by their cytotoxicity against ovarian, breast, and prostate cancer cell lines.

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