In-flow Real-time Detection of Spectrally Encoded Microgels for MiRNA Absolute Quantification

Overview

Journal Biomicrofluidics

Publisher American Institute of Physics

Specialty Biomedical Engineering

Date 2016 Dec 20

PMID 27990216

Citations 3

Authors

David Dannhauser

Filippo Causa

Edmondo Battista

Angela M Cusano

Domenico Rossi

Paolo A Netti

Affiliations

Soon will be listed here.

Abstract

We present an in-flow ultrasensitive fluorescence detection of microRNAs (miRNAs) using spectrally encoded microgels. We researched and employed a viscoelastic fluid to achieve an optimal alignment of microgels in a straight measurement channel and applied a simple and inexpensive microfluidic layout, allowing continuous fluorescence signal acquisitions with several emission wavelengths. In particular, we chose microgels endowed with fluorescent emitting molecules designed for multiplex spectral analysis of specific miRNA types. We analysed in a -real-time manner 80 microgel particles a minute at sample volumes down to a few microliters, achieving a miRNA detection limit of 202 fM in microfluidic flow conditions. Such performance opens up new routes for biosensing applications of particles within microfluidic devices.

Citing Articles

Unknown cell class distinction via neural network based scattering snapshot recognition.

Cioffi G, Dannhauser D, Rossi D, Netti P, Causa F Biomed Opt Express. 2023; 14(10):5060-5074.

PMID: 37854558 PMC: 10581789. DOI: 10.1364/BOE.492028.

Fluorescent microbeads for point-of-care testing: a review.

Zhang J, Shikha S, Mei Q, Liu J, Zhang Y Mikrochim Acta. 2019; 186(6):361.

PMID: 31101985 DOI: 10.1007/s00604-019-3449-y.

Bioengineering Microgels and Hydrogel Microparticles for Sensing Biomolecular Targets.

Battista E, Causa F, Netti P Gels. 2019; 3(2).

PMID: 30920517 PMC: 6318684. DOI: 10.3390/gels3020020.

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