A Comparison of Nanoparticle-antibody Conjugation Strategies in Sandwich Immunoassays

Overview

Journal J Immunoassay Immunochem

Specialty Allergy & Immunology

Date 2016 Dec 17

PMID 27982728

Citations 17

Authors

Justina O Tam

Helena de Puig

Chun-Wan Yen

Irene Bosch

Jose Gomez-Marquez

Charles Clavet

Kimberly Hamad-Schifferli

Lee Gehrke

Affiliations

Soon will be listed here.

Abstract

Point-of-care (POC) diagnostics such as lateral flow and dipstick immunoassays use gold nanoparticle (NP)-antibody conjugates for visual readout. We investigated the effects of NP conjugation, surface chemistries, and antibody immobilization methods on dipstick performance. We compared orientational, covalent conjugation, electrostatic adsorption, and a commercial conjugation kit for dipstick assays to detect dengue virus NS1 protein. Assay performance depended significantly on their conjugate properties. We also tested arrangements of multiple test lines within strips. Results show that orientational, covalent conjugation with PEG shield could improve NS1 detection. These approaches can be used to optimize immunochromatographic detection for a range of biomarkers.

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