Identification of Five Novel Typhi-Specific Genes As Markers for Diagnosis of Typhoid Fever Using Single-Gene Target PCR Assays

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2016 Dec 16

PMID 27975062

Citations 9

Authors

Yuan Xin Goay

Kai Ling Chin

Clarissa Ling Ling Tan

Chiann Ying Yeoh

Jaafar Nuhu Jaafar

Abdul Rahman Zaidah

Suresh Venkata Chinni

Kia Kien Phua

Affiliations

Soon will be listed here.

Abstract

Typhi (. Typhi) causes typhoid fever which is a disease characterised by high mortality and morbidity worldwide. In order to curtail the transmission of this highly infectious disease, identification of new markers that can detect the pathogen is needed for development of sensitive and specific diagnostic tests. In this study, genomic comparison of . Typhi with other enteric pathogens was performed, and 6 . Typhi genes, that is, STY0201, STY0307, STY0322, STY0326, STY2020, and STY2021, were found to be specific . Six PCR assays each targeting a unique gene were developed to test the specificity of these genes . The diagnostic sensitivities and specificities of each assay were determined using 39 . Typhi, 62 non-Typhi , and 10 non- clinical isolates. The results showed that 5 of these genes, that is, STY0307, STY0322, STY0326, STY2020, and STY2021, demonstrated 100% sensitivity (39/39) and 100% specificity (0/72). The detection limit of the 5 PCR assays was 32 pg for STY0322, 6.4 pg for STY0326, STY2020, and STY2021, and 1.28 pg for STY0307. In conclusion, 5 PCR assays using STY0307, STY0322, STY0326, STY2020, and STY2021 were developed and found to be highly specific at single-gene target resolution for diagnosis of typhoid fever.

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