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Selective Amplification of Tyv (rfbE), Prt (rfbS), ViaB, and FliC Genes by Multiplex PCR for Identification of Salmonella Enterica Serovars Typhi and Paratyphi A

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 2002 Feb 5
PMID 11825983
Citations 31
Authors
Kenji Hirose
Ken-ichiro Itoh
Hiroshi Nakajima
Takayuki Kurazono
Masanori Yamaguchi
Kazuo Moriya
Takayuki Ezaki
Yoshiaki Kawamura
Kazumichi Tamura
Haruo Watanabe
Affiliations
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Abstract

The PCR primers for O, H, and Vi antigen genes, tyv (rfbE), prt (rfbS), fliC-d, fliC-a, and viaB, were designed and used for the rapid identification of Salmonella enterica serovars Typhi and Paratyphi A with multiplex PCR. The results showed that all the clinical isolates examined of Salmonella serovars Typhi and Paratyphi A were accurately identified by this assay.

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