Encapsulation of Adenovirus BMP2-Transduced Cells with PEGDA Hydrogels Allows Bone Formation in the Presence of Immune Response

Overview

Journal Tissue Eng Part A

Specialties Anatomy & Histology
Biomedical Engineering
Biotechnology

Date 2016 Dec 15

PMID 27967655

Citations 1

Authors

Pedro Alvarez-Urena

Eleanor Davis

Corinne Sonnet

Gabrielle Henslee

Zbigniew Gugala

Edward V Strecker

Laura J Linscheid

Maude Cuchiara

Jennifer West

Alan Davis

Elizabeth Olmsted-Davis

Affiliations

Soon will be listed here.

Abstract

Gene therapy approaches have been difficult to implement due to pre-existing immunity against the virus used for delivery. To circumvent this problem, a cell-based approach was developed that avoided the use of free virus within the animal. However, even cells transduced in vitro with E1- to E3-deleted adenovirus encoding bone morphogenetic protein 2 (AdBMP2) resulted in the production of virus-neutralizing antibodies in mice. Furthermore, when mice received an intramuscular injection of nonencoding adenovirus (AdEmpty)-transduced cells, AdBMP2-transduced cells were unable to launch bone formation when an intramuscular injection of these BMP2-producing cells was delivered 1 week later. This phenomenon was not observed in NOD/SCID mice, and could be overcome in C57BL/6 mice by encapsulating the adenovirus-transduced cells in a nondegradable hydrogel poly(ethylene glycol) diacrylate (PEGDA). Data collectively suggest that PEGDA hydrogel encapsulation of AdBMP2-transduced cells prevents pre-existing immunity from suppressing BMP2-induced bone formation.

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Erfani A, Schieferstein J, Reichert P, Narasimhan C, Pastuskovas C, Parab V Adv Healthc Mater. 2023; 12(15):e2202370.

PMID: 36745878 PMC: 11469019. DOI: 10.1002/adhm.202202370.

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