Vitamin D Effects on Osteoblastic Differentiation of Mesenchymal Stem Cells from Dental Tissues

Overview

Journal Stem Cells Int

Publisher Wiley

Specialty Cell Biology

Date 2016 Dec 14

PMID 27956902

Citations 29

Authors

Francesca Posa

Adriana Di Benedetto

Graziana Colaianni

Elisabetta A Cavalcanti-Adam

Giacomina Brunetti

Chiara Porro

Teresa Trotta

Maria Grano

Giorgio Mori

Affiliations

Soon will be listed here.

Abstract

1,25-Dihydroxyvitamin D (1,25(OH)D), the active metabolite of vitamin D (Vit D), increases intestinal absorption of calcium and phosphate, maintaining a correct balance of bone remodeling. Vit D has an anabolic effect on the skeletal system and is key in promoting osteoblastic differentiation of human Mesenchymal Stem Cells (hMSCs) from bone marrow. MSCs can be also isolated from the immature form of the tooth, the dental bud: Dental Bud Stem Cells (DBSCs) are adult stem cells that can effectively undergo osteoblastic differentiation. In this work we investigated the effect of Vit D on DBSCs differentiation into osteoblasts. Our data demonstrate that DBSCs, cultured in an opportune osteogenic medium, differentiate into osteoblast-like cells; Vit D treatment stimulates their osteoblastic features, increasing the expression of typical markers of osteoblastogenesis like RUNX2 and Collagen I (Coll I) and, in a more important way, determining a higher production of mineralized matrix nodules.

Citing Articles

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